| Lack of ghrelin secretion in response to fasting in cholecystokinin-A (-1), -B (-2) receptor-deficient mice. | |
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MedLine Citation:
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PMID: 17134539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cholecystokinin receptors (CCK-Rs) have been classified into two subtypes: CCK-AR (1R) and -BR (2R). We generated CCK-AR(-/-), CCK-BR(-/-), and CCK-AR(-/-)BR(-/-) mice and found that the gastric emptying of a liquid meal was increased in CCK-BR(-/-) and AR(-/-)BR(-/-) mice, compared with wild-type and CCK-AR(-/-) mice. Given that enhanced gastric emptying leads to eating, food intake after overnight fasting was examined, as was the effect of CCK-8S on food intake. Male mice 6-8 months of age were deprived of food for 16 h with free access to water, after which they were injected intraperitoneally (0.1 ml/mouse) with either vehicle or CCK-8 (0.3, 1.0, or 3.0 nmol/mouse), and their food intake was monitored for 4 h. CCK-8S inhibited food intake in wild-type and CCK-BR(-/-) mice, but not in CCK-AR(-/-) or AR(-/-)BR(-/-) mice. Unexpectedly, we observed a lower food intake in CCK-AR(-/-)BR (-/-) mice treated with vehicle than in mice of the other genotypes. To examine the mechanism of decrease in food intake in CCK-AR(-/-)BR(-/-) mice, the involvement of ghrelin was determined in wild-type and CCK-AR(-/-)BR(-/-) mice. Fasting plasma ghrelin levels were significantly lower in CCK-AR (-/-)BR(-/-) mice than in wild-type mice, and no increase in response to fasting was observed in CCK-AR(-/-)BR(-/-) mice. An administration of acyl-ghrelin produced a small increase in food intake in CCK-AR(-/-)BR(-/-) mice, but not to the levels of wild-type mice. In conclusion, CCK-AR(-/-)BR(-/-) mice showed lower food intake as well as lower response to exogenous ghrelin, and a lower plasma ghrelin level after fasting, though which receptor is more important is unknown. |
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Authors:
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Chihiro Sakurai; Minoru Ohta; Setsuko Kanai; Hiroshi Uematsu; Akihiro Funakoshi; Kyoko Miyasaka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-01 |
Journal Detail:
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Title: The journal of physiological sciences : JPS Volume: 56 ISSN: 1880-6546 ISO Abbreviation: J Physiol Sci Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-12-27 Completed Date: 2007-04-03 Revised Date: 2008-12-29 |
Medline Journal Info:
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Nlm Unique ID: 101262417 Medline TA: J Physiol Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 441-7 Citation Subset: IM |
Affiliation:
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Department of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Tokyo, 173-0015 Japan. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dose-Response Relationship, Drug Eating / drug effects, physiology Fasting / metabolism* Gastric Emptying / physiology Ghrelin Male Mice Mice, Knockout Nootropic Agents / pharmacology Peptide Hormones / physiology, secretion* Receptor, Cholecystokinin A / genetics, metabolism* Receptor, Cholecystokinin B / genetics, metabolism* Sincalide / analogs & derivatives, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/8-sulfocholecystokinin octapeptide; 0/Ghrelin; 0/Nootropic Agents; 0/Peptide Hormones; 0/Receptor, Cholecystokinin A; 0/Receptor, Cholecystokinin B; 25126-32-3/Sincalide |
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