Document Detail


Lack of genoprotective effect of phytosterols and conjugated linoleic acids on Caco-2 cells.
MedLine Citation:
PMID:  19406198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Much interest has focused on the cholesterol-lowering effects of phytosterols (plant sterols) but limited data suggests they may also possess anti-carcinogenic activity. Conjugated linoleic acids (CLA), sourced from meat and dairy products of ruminant animals, has also received considerable attention as a potential anti-cancer agent. Therefore, the aims of this project were to (i) examine the effects of phytosterols and CLA on the viability and growth of human intestinal Caco-2 cells and (ii) determine their potential genoprotective (comet assay), COX-2 modulatory (ELISA) and apoptotic (Hoechst staining) activities. Caco-2 cells were supplemented with the phytosterols campesterol, beta-sitosterol, or beta-sitostanol, or a CLA mixture, or individual CLA isomers (c10t12-CLA, t9t11-CLA) for 48 h. The three phytosterols, at the highest levels tested, were found to reduce both the viability and growth of Caco-2 cells while CLA exhibited isomer-specific effects. None of the phytosterols protected against DNA damage. At a concentration of 25 microM, both c10t12-CLA and t9t11-CLA enhanced (P<0.05) oxidant-induced, but not mutagen-induced, DNA damage. Neither the phytosterols nor CLA induced apoptosis or modulated COX-2 production. In conclusion, campesterol, beta-sitosterol, beta-sitostanol, c10t12-CLA, and t9t11-CLA were not toxic to Caco-2 cells, at the lower levels tested, and did not exhibit potential anti-carcinogenic activity.
Authors:
Trevor J Daly; S Aisling Aherne; Tom P O'Connor; Nora M O'Brien
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-03
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  47     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-15     Completed Date:  2009-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1791-6     Citation Subset:  IM    
Affiliation:
Department of Food and Nutritional Sciences, University College Cork, Western Road, Cork, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Caco-2 Cells
Cell Membrane / drug effects,  enzymology
Cell Survival / drug effects
Cholesterol / analogs & derivatives,  pharmacology
Comet Assay
Cyclooxygenase 2 / biosynthesis
DNA Damage
Enzyme-Linked Immunosorbent Assay
Humans
Hydrogen Peroxide / antagonists & inhibitors,  toxicity
L-Lactate Dehydrogenase / metabolism
Linoleic Acid / pharmacology*
Methylnitronitrosoguanidine / toxicity
Mutagens / toxicity*
Phytosterols / pharmacology*
Protective Agents / pharmacology*
Sitosterols / pharmacology
Chemical
Reg. No./Substance:
0/Mutagens; 0/Phytosterols; 0/Protective Agents; 0/Sitosterols; 2197-37-7/Linoleic Acid; 474-62-4/campesterol; 57-88-5/Cholesterol; 5779-62-4/sitosterol; 70-25-7/Methylnitronitrosoguanidine; 7722-84-1/Hydrogen Peroxide; 83-45-4/stigmastanol; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.14.99.1/Cyclooxygenase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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