Document Detail

Lack of excitotoxic cell death in serum-free cultures of rat cerebral cortex.
MedLine Citation:
PMID:  1979519     Owner:  NLM     Status:  MEDLINE    
The excitotoxicity of glutamate, N-methyl-D-aspartate (NMDA), quisqualate and kainate were compared in rat cerebro-cortical cell cultures grown in different media. Excitotoxic cell death was examined by phase contrast microscopy and by the measurement of lactic dehydrogenase (LDH) activity released in the culture medium. Cells grown for 14 days in a serum-free, defined (N2) medium were resistant, while sister cultures maintained in the presence of serum responded to the excitotoxins by a significant degree of cell death. Cells cultured in serum-free medium for 7 days, and fed by a serum-containing one for an additional week exhibited vulnerability to the excitotoxins. It is concluded that the development of excitotoxicity in rat cortical cultures depends on one or more serum constituents.
S L Erdö; A Michler; J R Wolff; H Tytko
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  526     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1991-01-31     Completed Date:  1991-01-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  328-32     Citation Subset:  IM    
Department of Anatomy, Georg-August University, Göttingen, F.R.G.
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MeSH Terms
Amino Acids / toxicity*
Blood Physiological Phenomena
Cell Survival / drug effects
Cells, Cultured
Cerebral Cortex / cytology,  drug effects*
Culture Media / pharmacology
Glutamates / toxicity
Glutamic Acid
Kainic Acid / toxicity
L-Lactate Dehydrogenase / metabolism
N-Methylaspartate / toxicity
Quisqualic Acid / toxicity
Rats, Inbred Strains
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 0/Glutamates; 487-79-6/Kainic Acid; 52809-07-1/Quisqualic Acid; 56-86-0/Glutamic Acid; 6384-92-5/N-Methylaspartate; EC Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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