Document Detail

Lack of evidence for vesicle trafficking of fluorescent bile salts in rat hepatocyte couplets.
MedLine Citation:
PMID:  9124354     Owner:  NLM     Status:  MEDLINE    
The role of intracellular vesicles in the movement of bile salts through hepatocytes from blood to bile has not been resolved. To determine whether bile salts are sequestered during transit, rat hepatocyte couplets were incubated with the fluorescent bile salts cholyl-lysyl-fluorescein (CLF) and chenodeoxycholyl-lysyl-fluorescein (CDCLF). Cellular and canalicular fluorescence were measured by confocal scanning fluorescence microscopy; inhomogeneity in intracellular fluorescence was used to evaluate potential sequestering of bile salts. Mean cellular and canalicular fluorescence increased in parallel over 10 min, slightly exceeding (P < 0.05) the degree of increase in intracellular inhomogeneity. The microtubule inhibitor colchicine had no effect on cellular or canalicular fluorescence patterns. In contrast, the nonfluorescent bile salt taurocholate enhanced the recovery of microtubules from cold-induced depolymerization, measured by confocal immunofluorescence of beta-tubulin. Thus no evidence was obtained for intracellular sequestering of bile salts or microtubule-dependent trafficking before canalicular secretion; cellular uptake and distribution occurred in parallel with canalicular secretion. The previously documented dependence of bile salt secretion on intact microtubule function therefore appears to be an indirect rather than a direct consequence of microtubule-dependent events. In particular, enhanced microtubule assembly may play a role in bile salt-induced delivery of bile salt transporters to the canalicular membrane.
A Z El-Seaidy; C O Mills; E Elias; J M Crawford
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  272     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-04-24     Completed Date:  1997-04-24     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G298-309     Citation Subset:  IM    
Department of Pathology, Brigham and Women's Hospital, Harvard Digestive Diseases Center, and Harvard Medical School, Boston, Massachusetts 02115, USA.
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MeSH Terms
Bile Acids and Salts / antagonists & inhibitors,  metabolism*
Biological Transport
Chenodeoxycholic Acid / analogs & derivatives,  metabolism
Cholic Acids / metabolism
Fluoresceins / metabolism
Fluorescent Antibody Technique
Liver / cytology,  metabolism*
Microscopy, Confocal
Microscopy, Fluorescence
Microtubules / physiology
Rats, Sprague-Dawley
Grant Support
DK-34854/DK/NIDDK NIH HHS; DK-39512/DK/NIDDK NIH HHS; //Wellcome Trust
Reg. No./Substance:
0/Bile Acids and Salts; 0/Cholic Acids; 0/Fluoresceins; 0/cholyl-lysylfluorescein; 474-25-9/Chenodeoxycholic Acid

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