Document Detail


Lack of evidence for the involvement of rectal and oral trichomonads in the aetiology of vaginal trichomoniasis in Ghana.
MedLine Citation:
PMID:  16790560     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the possible involvement of human trichomonads (Pentatrichomonas hominis and Trichomonas tenax) other than Trichomonas vaginalis in the aetiology of vaginal trichomoniasis. METHODS: Vaginal swabs taken from women attending antenatal clinics were tested for Trichomonas vaginalis by traditional assays (wet-mount microscopy and InPouch culture) and nucleic acid amplification (polymerase chain reaction). These swabs were also tested for the presence of P hominis and T tenax by nucleic acid amplification. Oral and rectal swabs from these women were tested for T tenax and P hominis respectively. Data on sociodemographic characteristics, sexual and anogenital hygiene practices likely to seed P hominis and T tenax into the vagina were collected by a questionnaire. RESULTS: 93% (161) of the 173 samples in which T vaginalis was detected by wet preparation or culture was evaluable by PCR. Of this, T vaginalis was detected in 94% (152) by T vaginalis-specific PCR. Neither P hominis nor T tenax was detected in any of the vaginal swab samples. These included nine samples for which T vaginalis had been detected by wet preparation or culture, but were negative by T vaginalis nucleic acid amplification. P hominis and T tenax were not detected in any of the rectal and oral swabs, respectively. CONCLUSION: In this group of women, there was no evidence for the involvement of trichomonads other than T vaginalis in the aetiology of vaginal trichomoniasis.
Authors:
Y Adu-Sarkodie; B K Opoku; T Crucitti; H A Weiss; D Mabey
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-21
Journal Detail:
Title:  Sexually transmitted infections     Volume:  83     ISSN:  1368-4973     ISO Abbreviation:  Sex Transm Infect     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-16     Completed Date:  2007-05-25     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  9805554     Medline TA:  Sex Transm Infect     Country:  England    
Other Details:
Languages:  eng     Pagination:  130-2     Citation Subset:  IM    
Affiliation:
School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. sax01@tiscali.co.uk
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Animals
Female
Ghana / epidemiology
Humans
Mouth Diseases / epidemiology,  parasitology*
Polymerase Chain Reaction
Rectal Diseases / epidemiology,  parasitology*
Trichomonas / isolation & purification*
Trichomonas Vaginitis / epidemiology,  parasitology*
Vaginal Smears
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