Document Detail

Lack of effect of tumor necrosis factor-alpha -308 G/A polymorphism on severity of liver fibrosis in Tunisian hepatitis C virus (HCV)-infected patients.
MedLine Citation:
PMID:  20537485     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Tumor necrosis factor alpha (TNF-alpha) plays a key role in the immune response. An elevated plasma level of TNF-alpha was repeatedly observed in patients with active liver injury or cirrhosis regardless of the aetiology. The G/A transition at position -308 in the promoter region have been shown to influence TNF-alpha expression. In this study, we aimed to evaluate the impact of TNF-alpha -308 G/A functional polymorphism on fibrosis severity in Tunisian Hepatitis C Virus (HCV)-infected patients. METHODS: TNF-alpha -308 G/A polymorphism was evaluated by polymerase chain reaction (PCR) amplification followed by Restriction Fragment Length Polymorphism (RFLP) method in 53 chronic hepatitis C patients. Single-nucleotide polymorphism (SNP) frequencies were compared with regard to liver fibrosis severity as assessed by the METAVIR scoring system (F1-F2; n=22 versus F3-F4; n=31). RESULTS: The genotype distribution of the TNF-alpha -308 G/A polymorphism among the HCV-infected patients was as follows : GG : 67.9%, GA : 32.1%, AA : 0%. With regard to fibrosis score, no significant differences in TNF-alpha genotype distribution were observed between F1-F2 and F3-F4 patients (p=0.15). CONCLUSION: No significant association between TNF-alpha -308 polymorphism and and the severity of liver fibrosis was found in our Tunisian cohort.
N Bouzgarrou; E Hassen; S Gabbouj; E Schvoerer; N Ben Mami; H Triki; L Chouchane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-27
Journal Detail:
Title:  Gastroentérologie clinique et biologique     Volume:  34     ISSN:  2210-7401     ISO Abbreviation:  Gastroenterol. Clin. Biol.     Publication Date:    2010 Apr-May
Date Detail:
Created Date:  2010-07-12     Completed Date:  2010-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7704825     Medline TA:  Gastroenterol Clin Biol     Country:  France    
Other Details:
Languages:  eng     Pagination:  297-304     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Masson SAS. All rights reserved.
Molecular Immuno-Oncology Laboratory, Faculty of Medicine, 5019 Monastir, Tunisia.
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MeSH Terms
Case-Control Studies
Hepatitis C, Chronic / epidemiology*
Liver Cirrhosis / epidemiology*
Middle Aged
Polymorphism, Single Nucleotide*
Severity of Illness Index*
Tumor Necrosis Factor-alpha / blood,  genetics*
Tunisia / epidemiology
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha

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