Document Detail

Lack of effect of chronic hepatitis C virus infection on T-cell cytokine production in chronic hemodialysis patients.
MedLine Citation:
PMID:  11423688     Owner:  NLM     Status:  MEDLINE    
It has been shown that chronic hemodialysis modifies, to some extent, the normal immune response by both T and B lymphocytes elicited by antigenic stimulation, e.g. by impairing the T-cell-dependent response after vaccination. A new technique, i.e. flow cytometry, enables to assess intracytoplasmically, at the single cell level, the production of a given cytokine. By using it, we studied in healthy volunteers (HV) and in chronic hemodialysis (CHD) patients, with respect to their hepatitis C virus (HCV) status, the production by the T lymphocytes of type 1, and type 2 cytokines. We studied the following cytokines (CK): IL-2, IL-4, IL-5, IL-6, IL-10, IFN-gamma and TNF-alpha in the T-cell lymphocytes (whole, CD4+ and CD8+). There were 13 HV and 59 CHD patients (36 HCV(-) and 23 HCV(+)). Amongst the latter, there were 32 men and 27 women, aged 59.5 +/- 2 years, undergoing CHD since 70 +/- 9.4 months. We found that: (1) the total number of lymphocytes as well as those expressing CD3, CD4, or CD19 were significantly decreased in CHD patients as compared to those from HV; (2) the total number of lymphocytes as well as their different subsets were similar in HCV(+) and in HCV(-) CHD patients; (3) the frequency of T-cell-expressing IL-5 or IL-10 was always low (<1%) in both HV and CHD groups; (4) overall in CHD patients, the mean percentages of T lymphocytes expressing IL-2, IL-4, IFN-gamma or TNF-alpha were respectively 31 +/- 13, 2.5 +/- 1.3, 28 +/- 12 and 34 +/- 11% and were not statistically different between HCV(+) and HCV(-) patients; (5) IL-2 was mainly produced by CD4+ T cells, whereas IFN-gamma was produced by CD8+ T cells, in both HV and CHD groups, and (6) the lymphocytes of CHD patients produced significantly more IL-2 and IL-4 than those from HV, suggesting an activation of their T lymphocytes. We conclude that using the cytokine flow cytometry assay, our study demonstrated that in HCV(+) CHD patients, as opposed to what has been described for HCV(+) patients with normal renal function, there was no impairment in the production of type 1 cytokines by peripheral blood mononuclear cells when compared to HCV(-) CHD patients. Conversely to HV, T lymphocytes from CHD patients are activated.
L Rostaing; J S Borde; C Hasle; P Bories; A Allal; M Abbal; D Durand
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of nephrology     Volume:  21     ISSN:  0250-8095     ISO Abbreviation:  Am. J. Nephrol.     Publication Date:    2001 May-Jun
Date Detail:
Created Date:  2001-06-25     Completed Date:  2001-08-23     Revised Date:  2007-02-14    
Medline Journal Info:
Nlm Unique ID:  8109361     Medline TA:  Am J Nephrol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  194-9     Citation Subset:  IM    
Copyright Information:
Copyright 2001 S. Karger AG, Basel
Nephrology, Hemodialysis and Multiorgan Transplant Unit, Toulouse University Hospital, Toulouse, France.
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MeSH Terms
Cytokines / immunology*,  metabolism*
Flow Cytometry
Hepatitis C, Chronic / immunology*,  metabolism*
Kidney Failure, Chronic / immunology,  metabolism,  therapy
Middle Aged
Renal Dialysis*
T-Lymphocytes / immunology*,  metabolism*
Reg. No./Substance:

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