| Lack of a correlation between γH2AX foci kinetics in lymphocytes and the severity of acute normal tissue reactions during IMRT treatment for head and neck cancer. | |
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MedLine Citation:
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PMID: 21142613 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To investigate the phosphorylated histone H2A isoform X (γH2AX) foci kinetics as an indicator for the development of acute normal tissue complications during Intensity-Modulated Radiotherapy (IMRT) for head and neck cancer (HNC) patients. MATERIALS AND METHODS: Microscopic scoring of the γH2AX foci was used to evaluate the DNA-double-strand-break repair capacity in from Ataxia-Telangiectasia (A-T) patients derived lymphoblastoid cell lines (LCL) and T-lymphocytes isolated from 31 IMRT treated HNC patients. Cells were irradiated in vitro with 0.5 Gy given at high-dose-rate (HDR) and examined at several times up to 24 h after irradiation. The patients were subdivided in three groups showing mild, moderate and severe acute normal tissue complications based on their Common Toxicity Criteria grades for dysphagia, mucositis and dermatitis during the radiotherapy course. RESULTS: For the ATM (Ataxia-Telangiectasia-Mutated) defective LCL, a lower number of radiation-induced foci and a somewhat less efficient repair capacity was observed. No correlation was found between the γH2AX foci kinetics pattern and the risk for acute normal tissue complications among the three patient subgroups. CONCLUSIONS: Scoring of γH2AX foci after in vitro irradiation of isolated T-lymphocytes of HNC patients cannot be applied to predict for the development of acute normal tissue complications. |
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Authors:
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Joke Werbrouck; Fréderic Duprez; Wilfried De Neve; Hubert Thierens |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of radiation biology Volume: 87 ISSN: 1362-3095 ISO Abbreviation: Int. J. Radiat. Biol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-14 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: England |
Other Details:
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Languages: eng Pagination: 46-56 Citation Subset: IM; S |
Affiliation:
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Department of Basic Medical Sciences, Ghent University, Gent, Belgium. joke.werbrouck@ugent.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Carcinoma, Squamous Cell / metabolism, pathology, radiotherapy Cell Line DNA Breaks, Double-Stranded DNA Repair Female Gamma Rays / adverse effects Head and Neck Neoplasms / metabolism*, pathology, radiotherapy* Histones / metabolism* Humans Kinetics Lymphocytes / metabolism* Male Middle Aged Predictive Value of Tests Radiation Injuries / etiology, metabolism, pathology Radiation Tolerance Radiotherapy, Intensity-Modulated* / adverse effects |
| Chemical | |
Reg. No./Substance:
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0/H2AFX protein, human; 0/Histones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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