Document Detail


Lack of cell cycle regulation of telomerase activity in human cells.
MedLine Citation:
PMID:  9380696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Conflicting reports have appeared concerning the cell cycle regulation of telomerase activity and its possible repression during quiescence and cell differentiation. We have reexamined these issues in an attempt to uncover the basis for the discrepancies. Variations in extracted telomerase activity during the cell cycle are not observed in cells sorted on the basis of DNA content. Variations are observed in cells synchronized using some biochemical cell cycle inhibitors, but only with those agents where cellular toxicity is evident. A progressive decline in telomerase activity is observed in cells whose growth rate is reduced from seven to eight population doublings per week to one to two doublings per week. Telomerase is largely absent in cells that truly exit the cell cycle and do not divide over the 7-day period. Although it is not necessary for all cell types to regulate telomerase in the same way, we conclude that in the immortal cultured cell lines examined, extracted telomerase activity does not change significantly during progression through the stages of the cell cycle. Telomerase activity generally correlates with growth rate and is repressed in cells that exit the cell cycle and become quiescent.
Authors:
S E Holt; D L Aisner; J W Shay; W E Wright
Related Documents :
18468396 - Dendroflorin retards the senescence of mrc-5 cells.
20833136 - Reduction of nup107 attenuates the growth factor signaling in the senescent cells.
18997296 - Blueberry antagonism of c-2 ceramide disruption of ca2+ responses and recovery in machr...
8735906 - In vitro senescence enhances il-6 production in human gingival fibroblasts induced by l...
17057226 - Temporal and spatial variations of lipid droplets during adipocyte division and differe...
20583256 - Hepatocyte growth factor induces invasion and migration of ovarian cancer cells by decr...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  94     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-11-10     Completed Date:  1997-11-10     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10687-92     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Neuroscience, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75235-9039, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Cycle* / drug effects
Cell Line, Transformed
Flow Cytometry
Half-Life
Humans
Telomerase / metabolism*
Grant Support
ID/Acronym/Agency:
AG01228/AG/NIA NIH HHS; AG05747/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
EC 2.7.7.49/Telomerase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Iron regulatory protein 1 is not required for the modulation of ferritin and transferrin receptor ex...
Next Document:  Two sterol regulatory element-like sequences mediate up-regulation of caveolin gene transcription in...