| Lack of catecholamine involvement in the increased luteinizing hormone release due to blockade of kappa-opioid receptors in the medial basal hypothalamus during midpregnancy in the rat. | |
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MedLine Citation:
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PMID: 8032878 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Blockade of kappa-opioid receptors in the medial basal hypothalamus (MBH) with nor-binaltorphimine (nor-BNI) stimulates luteinizing hormone (LH) release during midpregnancy in the rat [48]. The objective of this study was to determine whether norepinephrine (NE) or dopamine (DA) mediates the LH response to blockade of MBH kappa-opioid receptors on days 13-17 of pregnancy in the rat. Two experiments were conducted. In the first, push-pull perfusion in conjunction with HPLC was used to monitor in vivo NE release in the MBH occurring in response to (a) artificial CSF followed by CSF containing nor-BNI (40 micrograms/h), (b) desipramine (DMI, a NE reuptake blocker, 10 microM) in CSF followed by DMI, and (c) DMI followed by DMI+nor-BNI. Blood samples were taken at 12 min intervals concurrent with push-pull perfusate samples. Plasma LH levels were determined by RIA. Nor-BNI significantly increased LH release compared to CSF alone, but perfusate NE was undetectable in either perfusion period. However, perfusion with CSF containing 100 mM K+ in these rats markedly increased perfusate NE levels, indicating noradrenergic nerve terminals were present at the perfusion sites in the MBH. Addition of DMI to the CSF significantly increased perfusate NE levels, but produced no change in LH release. Nor-BNI+DMI perfusion increased LH secretion similar to nor-BNI alone, but produced no additional increase in MBH perfusate NE levels compared to perfusion with DMI alone.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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S Zhen; R V Gallo |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research Volume: 642 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1994 Apr |
Date Detail:
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Created Date: 1994-08-16 Completed Date: 1994-08-16 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 178-84 Citation Subset: IM |
Affiliation:
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Department of Physiology and Neurobiology, University of Connecticut, Storrs 06269-4154. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dopamine / physiology* Female Hypothalamus, Middle / drug effects*, secretion Luteinizing Hormone / secretion* Naltrexone / analogs & derivatives, pharmacology Norepinephrine / physiology* Perfusion Pregnancy Pregnancy, Animal / metabolism* Rats Rats, Sprague-Dawley Receptors, Opioid, kappa / antagonists & inhibitors* Secretory Rate / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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HD-17728/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Opioid, kappa; 105618-26-6/norbinaltorphimine; 16590-41-3/Naltrexone; 51-41-2/Norepinephrine; 9002-67-9/Luteinizing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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