Document Detail

Lack of association between the TGF-beta1 gene polymorphisms and recurrent spontaneous abortion.
MedLine Citation:
PMID:  16236363     Owner:  NLM     Status:  MEDLINE    
Transforming growth factor-beta1 (TGF-beta1) is produced by T regulatory lymphocytes (Treg), which play an important role in the physiology of pregnancy. Several polymorphisms of the TGF-beta1 gene (TGFB1) have been reported, some with an important correlation with TGF-beta1 production and disease severity. We performed an association study between TGFB1 polymorphisms and recurrent spontaneous abortion (RSA). We first used a PCR-RFLP method to detect three known TGFB1 cSNPs (coding single nucleotide polymorphisms) among 111 RSA and 110 normal control women from Southern Iran, such as 29T-->C (Leu 10 Pro), 74G-->C (Arg 25 Pro) and 788C-->T (Thr 263Ile), and compared their frequencies between the two groups of subjects. To confirm results of the RFLP study and to identify new SNPs in the RSA women, we then sequenced their DNA samples for seven exons and adjacent intronic regions of TGFB1. Consequently, 10 SNPs were detected; one (-14G-->A) was located in the upstream region of exon 1, three in exons (two in exon 1 and one in exon 5) and six in intronic regions. Two (IVS5+18G-->C and IVS6+910G-->A) of the 10 SNPs were novel. Statistical analysis on the frequency of six most frequent SNPs, including the three cSNPs, as well as on the frequencies of genotypes and 13 haplotypes regarding the 6 SNPs, revealed no significant difference between RSA and control women. Therefore, this study concludes that there is no association between exonic and adjacent intronic polymorphisms of TGFB1 and RSA.
Dawar Amani; Alamtaj Samsami Dehaghani; Jaleh Zolghadri; Fatemeh Ravangard; Norrio Niikawa; Ko-ichiro Yoshiura; Abbas Ghaderi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-19
Journal Detail:
Title:  Journal of reproductive immunology     Volume:  68     ISSN:  0165-0378     ISO Abbreviation:  J. Reprod. Immunol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-06     Completed Date:  2006-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8001906     Medline TA:  J Reprod Immunol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  91-103     Citation Subset:  IM    
Department of Immunology, Medical School, Ardabil University of Medical Sciences, Ardabil, Iran.
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MeSH Terms
Abortion, Habitual / genetics*,  immunology
Exons / genetics,  immunology
Introns / genetics,  immunology
Middle Aged
Polymorphism, Single Nucleotide / genetics*,  immunology
Predictive Value of Tests
T-Lymphocytes, Regulatory / immunology
Transforming Growth Factor beta / genetics*,  immunology
Transforming Growth Factor beta1
Reg. No./Substance:
0/TGFB1 protein, human; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1

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