| Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. | |
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MedLine Citation:
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PMID: 16868779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Oxidative damage to the retinal pigment epithelium might be involved in the pathogenesis of age related macular degeneration. Thus antioxidative protection represents a rationale for a causative therapy or prophylaxis. The aim of the present study is to evaluate antioxidative properties of vitamin C and pyruvate at retinal pigment epithelial (RPE) cells exposed to oxidative stress. METHODS: The ability of vitamin C and pyruvate to quench hydroxyl radicals was tested using the di-hydro-rhodamine (DHR) assay. Cells of the human RPE cell line ARPE-19 were exposed for 8 min to hydroxyl radicals generated by the Fenton reaction from 2.25 mM H2O2 and 30 microM Fe3+ -nitrilo-tri-acetate. This was done in the absence and presence of 0.3-3.0 mM pyruvate and vitamin C, respectively. Cell survival was analysed by vitality staining (life-dead-assay) and expressed as cell survival ratio. A survival ratio <1.0 indicates cell loss. RESULTS: At concentrations from 0.1 to 1.0 mM vitamin C and pyruvate quench hydroxyl radicals in the DHR assay in absence of living matter. In the presence of 0.1- 0.3 mM vitamin C and pyruvate, ARPE-19 showed a reduced survival ratio (0.87 +/- 0.01 to 0.89 +/- 0.02 after 6 h) which was not the case at the higher concentrations between 1 and 3 mM. The exposure of ARPE-19 cells to hydroxyl radicals reduced the survival ratio to 0.92 +/- 0.02. At concentrations at which vitamin C and pyruvate exert toxic effects, a potentiation of radical induced cell death can be observed (survival ratio 0.79 +/- 0.02 and 0.82 +/- 0.03, respectively). Higher concentrations of vitamin C or pyruvate had no explicit protective effect to the hydroxyl radical induced damage. DISCUSSION: Although vitamin C and pyruvate are potent hydroxyl radical quenchers in vitro they failed to protect cultured ARPE-19 cells from oxidative stress induced cell death. In contrast, when applying the scavengers at low concentrations a potentiation of cell damage was observed. |
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Authors:
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Oliver Zeitz; Lars Schlichting; Gisbert Richard; Olaf Strauss |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Volume: 245 ISSN: 0721-832X ISO Abbreviation: Graefes Arch. Clin. Exp. Ophthalmol. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-10-23 Completed Date: 2007-12-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205248 Medline TA: Graefes Arch Clin Exp Ophthalmol Country: Germany |
Other Details:
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Languages: eng Pagination: 276-81 Citation Subset: IM |
Affiliation:
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Universitätsklinikum Hamburg-Eppendorf, Klinik und Poliklinik ffir Augenheilkunde, Martinistr. 52, 20246 Hamburg, Germany. zeitz@uke.uni-hamburg.de |
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology* Ascorbic Acid / pharmacology* Cell Survival Cells, Cultured Free Radical Scavengers / pharmacology Humans Hydrogen Peroxide / toxicity Hydroxyl Radical Oxidative Stress / drug effects Pigment Epithelium of Eye / cytology, drug effects* Pyruvic Acid / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Free Radical Scavengers; 127-17-3/Pyruvic Acid; 3352-57-6/Hydroxyl Radical; 50-81-7/Ascorbic Acid; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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