Document Detail

Lack of Na(+),K (+)-ATPase expression in intercalated cells may be compensated by Na(+)-ATPase: a study on MDCK - C11 cells.
MedLine Citation:
PMID:  18759127     Owner:  NLM     Status:  MEDLINE    
The lack of Na(+),K(+)-ATPase expression in intercalated cells (IC) is an intriguing condition due to its fundamental role in cellular homeostasis. In order to better understand this question we compared the activities of Na(+),K(+)-ATPase and Na(+)-ATPase in two MDCK cell clones: the C11, with IC characteristics, and the C7, with principal cells (PC) characteristics. The Na(+),K(+)-ATPase activity found in C11 cells is far lower than in C7 cells and the expression of its beta-subunit is similar in both cells. On the other hand, a subset of C11 without alpha-subunit expression has been found. In C11 cells the Na(+)-ATPase activity is higher than that of the Na(+),K(+)-ATPase, and it is increased by medium alkalinization, suggesting that it could account for the cellular Na(+)-homeostasis. Although further studies are necessary for a better understanding of these findings, the presence of Na(+)-ATPase may explain the adequate survival of cells that lack Na(+),K(+)-ATPase.
M S Sampaio; I P Bezerra; F L Peçanha; P H Fonseca; M A M Capella; A G Lopes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  65     ISSN:  1420-682X     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-30     Completed Date:  2008-11-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  3093-9     Citation Subset:  IM    
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
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MeSH Terms
Adenosine Triphosphatases / metabolism*
Cation Transport Proteins / metabolism*
Cell Line
Fluorescent Dyes / metabolism
Nephrons / cytology*
Protein Subunits / metabolism
Sodium / metabolism
Sodium-Potassium-Exchanging ATPase / metabolism*
Reg. No./Substance:
0/Cation Transport Proteins; 0/Fluorescent Dyes; 0/Protein Subunits; 7440-23-5/Sodium; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/sodium-translocating ATPase; EC ATPase

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