Document Detail

Lack of HCV infection in malignant cells refutes the hypothesis of a direct transforming action of the virus in the pathogenesis of HCV-associated B-cell NHLs.
MedLine Citation:
PMID:  12487559     Owner:  NLM     Status:  MEDLINE    
AIMS AND BACKGROUND: Preliminary evidence suggests that hepatitis C virus (HCV) might play a pathogenetic role in autoimmune-related, non-malignant B-cell lymphoproliferation, as well as in a subset of B-cell non-Hodgkin's lymphomas (NHLs). With regard to the mechanism(s) by which HCV might favor B-cell expansion and malignant transformation, most data support an indirect pathogenetic role of the virus as an exogenous trigger. A direct oncogenetic role of HCV by direct cell infection and deregulation has only been hypothesized on the basis of the lymphotropism of the virus. METHODS: In this study we investigated the possible HCV infection of NHL B cells by means of sensitive and quantitative polymerase chain reaction (PCR) on affinity-purified neoplastic cells, and by HCV-specific immunohistochemistry and in situ hybridization. RESULTS: HCV infection of neoplastic B cells was documented in only three cases, namely the low-grade B-cell NHLs that arose in the course of mixed cryoglobulinemia syndrome (MC). HCV infection, below one viral genome per cell, was detectable only by PCR. All the remaining low-grade (one case) and high-grade B-cell NHLs (two cases) were HCV uninfected. Previous immunoglobulin gene analyses were consistent with an antigen-driven B-cell lymphoproliferation in the studied cases. CONCLUSIONS: Overall, our data are consistent with an indirect oncogenetic role of HCV in B-cell lymphomagenesis as an exogenous trigger. Infection of B cells by HCV appears possible in some NHL subsets, but the implications remain unknown.
Salvatore De Vita; Valli De Re; Domenico Sansonno; Annunziata Gloghini; Daniela Gasparotto; Massimo Libra; Stefania Sacco; Antonino Carbone; Gianfranco Ferraccioli; Mauro Boiocchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tumori     Volume:  88     ISSN:  0300-8916     ISO Abbreviation:  Tumori     Publication Date:    2002 Sep-Oct
Date Detail:
Created Date:  2002-12-18     Completed Date:  2003-01-10     Revised Date:  2008-12-12    
Medline Journal Info:
Nlm Unique ID:  0111356     Medline TA:  Tumori     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  400-6     Citation Subset:  IM    
Division of Rheumatology, DPMSC, University of Udine, Italy.
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MeSH Terms
B-Lymphocytes / pathology*,  virology*
Cell Transformation, Neoplastic
Cell Transformation, Viral
Flow Cytometry
Hepacivirus / genetics,  isolation & purification*
Hepatitis C / complications*
In Situ Hybridization
Lymphoma, B-Cell / virology*
Middle Aged
Polymerase Chain Reaction
RNA, Viral / isolation & purification
Reg. No./Substance:
0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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