| Laboratory diagnosis of endophthalmitis: comparison of microbiology and molecular methods in the European Society of Cataract & Refractive Surgeons multicenter study and susceptibility testing. | |
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MedLine Citation:
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PMID: 18721702 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To investigate and compare the use of molecular biology with the use of traditional Gram stain and organism culture for the laboratory diagnosis of postoperative endophthalmitis. SETTING: Twenty-four ophthalmology units together with 9 microbiology laboratories and 2 European reference molecular biology laboratories. METHODS: A prospective randomized partially masked multicenter cataract surgery study recruited 16 603 patients. This resulted in 29 cases of presumed postoperative endophthalmitis. Gram stain and culture were performed in the local laboratory according to agreed protocols. Samples of aqueous and/or vitreous were transported to the first referenced molecular biology laboratory (Regensburg, Germany) for polymerase chain reaction (PCR) testing, and an extracted aliquot of DNA was then referred to the second laboratory (Alicante, Spain) for PCR. RESULTS: Of the 29 who presented with presumed postoperative endophthalmitis, 20 were classified as proven infective endophthalmitis with positive Gram stain, culture, or PCR. Fourteen patients were culture-positive; all but 1 of these was also positive by PCR. Six patients were positive by PCR but negative by Gram stain or culture. Nine patients were negative by both microbiology and PCR testing. CONCLUSIONS: Use of molecular biology technique increased the laboratory rate of identifying the pathogen by 20%, confirming the technique is very useful for the endophthalmitis specimen. Samples of both aqueous and vitreous should be collected and stored at -20 degrees C for PCR at the time of the diagnostic taps. |
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Authors:
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David Seal; Udo Reischl; Anke Behr; Consuelo Ferrer; Jorge Alió; Roland J Koerner; Peter Barry; |
Publication Detail:
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Type: Comparative Study; Journal Article; Multicenter Study; Randomized Controlled Trial |
Journal Detail:
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Title: Journal of cataract and refractive surgery Volume: 34 ISSN: 0886-3350 ISO Abbreviation: J Cataract Refract Surg Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-25 Completed Date: 2008-10-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8604171 Medline TA: J Cataract Refract Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1439-50 Citation Subset: IM |
Affiliation:
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Applied Vision Research Centre, City University, London, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aqueous Humor
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microbiology Bacteria / genetics, isolation & purification Bacterial Typing Techniques / methods* DNA, Bacterial / analysis Endophthalmitis / diagnosis*, microbiology Europe Eye Infections, Bacterial / diagnosis*, microbiology Gentian Violet Humans Microbial Sensitivity Tests Ophthalmology / standards Phacoemulsification Phenazines Polymerase Chain Reaction / methods* Postoperative Complications / diagnosis*, microbiology Prospective Studies Societies, Medical / standards Vitreous Body / microbiology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Gram's stain; 0/Phenazines; 548-62-9/Gentian Violet |
| Investigator | |
Investigator/Affiliation:
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Peter Barry / ; David Seal / ; George Gettinby / ; Magnus Peterson / ; Crawford Revie / ; Fiona Lees / ; Klaus Geldsetzer / ; David Lloyd / ; Gunther Grabner / ; Stefan F Egger / ; Josef Ruckhofer / ; Johan Blanckaert / ; Jan Yperman Ziekenhuis / ; Camille Budo / ; Albert Galand / ; Jessica Crommen / ; Jean Rakic / ; Gaël Xhauflaire / ; Marie-José Tassignon / ; Hugo Verbraeken / ; Rita de Donker / ; Stefanie Schmickler / ; Roberto Bellucci / ; Simonetta Morselli / ; Sandro Soldati / ; Fausto Vigasio / ; Marco Bertelli / ; Andrea Bottoli / ; Marta Cassamali / ; Fabrizio Danieli / ; Samer Khuri / ; Luigina Rosa / ; Jerzy Szaflik / ; Jacek P Szaflik / ; Conceição Lobo / ; José Cunha-Vaz / ; Joaquin Mira / ; Augusto Abreu / ; José Aguilar / ; Victor Arteaga / ; Luis Cordovés / ; Valentín T Díaz-Alemán / ; Manuel Gonzalez de la Rosa / ; Cristina Mantolan / ; Pedro Abreu / ; Jorge Alvarez-Marín / ; María Antonia Gil / ; Jorge Alió / ; Miguel Teus / ; M T Alvarez / ; J M Román / ; Süleyman Kaynak / ; Osman Saatcy / ; Ysmet Durak / ; Uzeyir Günenç / ; Tülin Berk / ; Meltem Söylev / ; Hakan Oner / ; Nilüfer Koçak / ; Mehmet Ergin / ; David Allen / ; Peter Phelan / ; David Steel / ; Chris Wood / ; Carol Cunningham / ; Michael Miller / ; Ramesh Moorthy / ; Andeeb Zafar / ; Alex Ionides / ; Damian Lake / ; Graham Thompson / ; John Jacob / ; Daniel Bytes / ; Casper Gibbon / ; Andrew Kleinschmidt / ; Roland Ling / ; Anthony Quinn / ; Peter Simcock / ; George Sturrock / ; William Kiel / ; Denise Mabey / ; David Spalton / ; Anupma Kumar / ; Paul Rosen / ; C K Patel / ; John Salmon / ; Jean Dash / ; Linda Lindsell / ; Rebecca Turner / ; Susan Kennedy / ; Per Montan / ; Stephen Barrett / ; John Ludgate / ; Susanne Gardner / ; Kirk Wilhelmus / |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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