Document Detail


Laboratory diagnosis of Mycoplasma pneumoniae infection. 3. Detection of IgM antibodies to M. pneumoniae by a modified indirect haemagglutination test.
MedLine Citation:
PMID:  2514114     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The indirect haemagglutination (IHA) test was compared with the complement-fixation (CF) test for the measurement of antibodies to Mycoplasma pneumoniae. A modification of the IHA was used to measure M. pneumoniae IgM antibodies. Sera were obtained from various groups of patients who were either culture or antigen positive for M. pneumoniae in nasopharyngeal aspirates or who had fourfold or greater increase in CF antibody or a titre greater than or equal to 320. The results of these comparisons showed that the modified IHA test was specific and more sensitive (89% as opposed to 64%) than the CF test. The modified IHA test for the detection of IgM antibody was highly effective in the recognition of recent or current infection with the mycoplasma. It was also of equal sensitivity to an indirect enzyme immunoassay for the detection of IgM antibodies to M. pneumoniae.
Authors:
T W Kok; B P Marmion; G Varkanis; D A Worswick; J Martin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Epidemiology and infection     Volume:  103     ISSN:  0950-2688     ISO Abbreviation:  Epidemiol. Infect.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-02-22     Completed Date:  1990-02-22     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  8703737     Medline TA:  Epidemiol Infect     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  613-23     Citation Subset:  IM    
Affiliation:
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Bacterial / analysis*
Complement Fixation Tests
Hemagglutination Tests
Humans
Immunoenzyme Techniques
Immunoglobulin M / analysis*
Mycoplasma pneumoniae / immunology*
Nasopharynx / microbiology
Pneumonia, Mycoplasma / diagnosis*
Predictive Value of Tests
Rheumatoid Factor / analysis
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Immunoglobulin M; 9009-79-4/Rheumatoid Factor
Comments/Corrections

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