Document Detail


Labelling of polysaccharides using [11C]cyanogen bromide. In vivo and in vitro evaluation of 11C-hyaluronan uptake kinetics.
MedLine Citation:
PMID:  7539321     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for the 11C-labelling of polysaccharides in high specific radioactivity is described. Dextran and hyaluronan were treated with [11C]cyanogen bromide in aqueous solution at pH 11.5 to give 30-47% radiochemical yields with higher than 98% radiochemical purity in synthesis times of 24-26 min counted from the end of bombardment. Specific radioactivities at the end of synthesis ranged from 0.12 to 3.1 Ci/mumol. The biodistribution kinetics of [11C]hyaluronan injected intravenously was studied in rats by means of positron emission tomography, showing a rapid and displaceable uptake in liver. Uptake and displacement of [11C]hyaluronan was also demonstrated in cultured rat liver endothelial cells.
Authors:
G Westerberg; M Bergström; S Gustafson; U Lindqvist; A Sundin; B Långström
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nuclear medicine and biology     Volume:  22     ISSN:  0969-8051     ISO Abbreviation:  Nucl. Med. Biol.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-07-06     Completed Date:  1995-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9304420     Medline TA:  Nucl Med Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  251-6     Citation Subset:  IM    
Affiliation:
Uppsala University PET Centre, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Carbon Radioisotopes / pharmacokinetics*
Cells, Cultured
Chromatography, High Pressure Liquid
Cyanogen Bromide
Dextrans / chemistry,  pharmacokinetics*
Hyaluronic Acid / chemistry,  pharmacokinetics*
Indicators and Reagents
Isotope Labeling / methods
Kinetics
Liver / metabolism*,  radionuclide imaging
Male
Myocardium / metabolism
Rats
Rats, Sprague-Dawley
Time Factors
Tomography, Emission-Computed / methods
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Indicators and Reagents; 506-68-3/Cyanogen Bromide; 9004-54-0/Dextrans; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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