Document Detail


Labelling of a T lymphocyte subpopulation with monoclonal antibody bound to colloidal gold. Correlation with the indirect immunofluorescence method.
MedLine Citation:
PMID:  3071478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method of direct labelling of human lymphocytes CD8 with a monoclonal antibody bound to colloidal gold particles is described. Results of labelling of isolated lymphocytes and of cells of leucocyte concentrate from haemolysed blood correlated well with those obtained by indirect immunofluorescence. Owing to its simplicity and rapidity, the described method is particularly suitable for clinical diagnostics.
Authors:
L Korcáková; J Sálková; J Lejnar; J Kaslík; I Raska
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia biologica     Volume:  34     ISSN:  0015-5500     ISO Abbreviation:  Folia Biol. (Praha)     Publication Date:  1988  
Date Detail:
Created Date:  1989-05-16     Completed Date:  1989-05-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0234640     Medline TA:  Folia Biol (Praha)     Country:  CZECHOSLOVAKIA    
Other Details:
Languages:  eng     Pagination:  209-14     Citation Subset:  IM    
Affiliation:
Institute of Clinical and Experimental Medicine, Praha.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Colloids
Fluorescent Antibody Technique
Gold
Humans
T-Lymphocytes / classification*,  immunology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Colloids; 7440-57-5/Gold

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