| Labeling of skeletal myoblasts with a novel oxygen-sensing spin probe for noninvasive monitoring of in situ oxygenation and cell therapy in heart. | |
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MedLine Citation:
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PMID: 17142337 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report the labeling (internalization) of skeletal myoblasts (SMs) with a novel class of oxygen-sensing paramagnetic spin probe for noninvasive tracking and in situ monitoring of oxygenation in stem cell therapy using electron paramagnetic resonance (EPR) spectroscopy. SM cells were isolated from thigh muscle biopsies of mice and propagated in culture. Labeling of SM cells with the probe was achieved by coincubating the cells with submicron-sized (270 +/- 120 nm) particulates of the probe in culture for 48 h. The labeling had no significant effect on the viability or proliferation of the cells. The SM cells labeled with the probe were transplanted in the infarcted region of mouse hearts. The engraftment of the transplanted cells in the infarct region was verified by using MY-32 staining for skeletal myocytes. The in situ Po(2) in the heart was determined noninvasively and repeatedly for 4 wk after transplantation. The results showed significant enhancement of myocardial oxygenation at the site of cell transplant compared with untreated control. In conclusion, labeling of SM cells with the oxygen-sensing spin probe offers a unique opportunity for the noninvasive monitoring of transplanted cells as well as in situ tissue Po(2) in infarcted mouse hearts. |
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Authors:
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Sheik Wisel; Simi M Chacko; M Lakshmi Kuppusamy; Ramasamy P Pandian; Mahmood Khan; Vijay Kumar Kutala; Richard W Burry; Benjamin Sun; Pawel Kwiatkowski; Periannan Kuppusamy |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-12-01 |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 292 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-03-06 Completed Date: 2007-04-26 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H1254-61 Citation Subset: IM |
Affiliation:
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Center for Biomedical Electron Paramagnetic Resonance Spectroscopy and Imaging, Division of Cardiothoracic Surgery, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques Cell Survival Electron Spin Resonance Spectroscopy Flow Cytometry Heart / physiology*, physiopathology* Mice Models, Animal Muscle, Skeletal / cytology, physiology* Myocardial Infarction / physiopathology* Myocardium / cytology Oxygen / analysis* Tissue Therapy / methods* |
| Grant Support | |
ID/Acronym/Agency:
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R01-EB-004031/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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