| Labeling membranes with fluorescent cholesterols. | |
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MedLine Citation:
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PMID: 21536756 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: Cholesterol, an essential component of most cellular membranes, is present in the plasma membrane and is enriched within lipid rafts. It is, however, virtually absent from the mitochondrial inner membrane, and there is little within the endoplasmic reticulum. Dehydroergosterol (DHE), a fluorescent cholesterol analog, has been found to effectively label membranes and has been used to monitor cholesterol distribution and trafficking within live cells. Other cholesterol-based probes, such as NBD (N-(7-nitrobenz-2-oxa-1,3-diazole-4-yl))-cholesterol, are commercially available (from Molecular Probes). However, cell labeling using the NBD-cholesterol analogs frequently does not show the same labeling pattern as for DHE and native cholesterol. |
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Authors:
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Brad Chazotte |
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Publication Detail:
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Type: Journal Article Date: 2011-05-01 |
Journal Detail:
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Title: Cold Spring Harbor protocols Volume: 2011 ISSN: 1559-6095 ISO Abbreviation: Cold Spring Harb Protoc Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-05-03 Completed Date: 2011-08-09 Revised Date: 2012-01-18 |
Medline Journal Info:
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Nlm Unique ID: 101524530 Medline TA: Cold Spring Harb Protoc Country: United States |
Other Details:
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Languages: eng Pagination: pdb.prot5624 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Azoles
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metabolism Cell Membrane / metabolism* Cytological Techniques / methods* Ergosterol / analogs & derivatives*, metabolism Fluorescent Dyes / metabolism* Nitrobenzenes / metabolism Staining and Labeling / methods* |
| Chemical | |
Reg. No./Substance:
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0/7-nitrobenz-2-oxa-1,3-diazol-4-yl; 0/Azoles; 0/Fluorescent Dyes; 0/Nitrobenzenes; 516-85-8/dehydroergosterol; 57-87-4/Ergosterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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