| Labeling human embryonic stem-cell-derived cardiomyocytes for tracking with MR imaging. | |
| | |
MedLine Citation:
|
PMID: 21594541 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: Human embryonic stem cells (hESC) can generate cardiomyocytes (CM), which offer promising treatments for cardiomyopathies in children. However, challenges for clinical translation result from loss of transplanted cell from target sites and high cell death. An imaging technique that noninvasively and repetitively monitors transplanted hESC-CM could guide improvements in transplantation techniques and advance therapies. OBJECTIVE: To develop a clinically applicable labeling technique for hESC-CM with FDA-approved superparamagnetic iron oxide nanoparticles (SPIO) by examining labeling before and after CM differentiation. MATERIALS AND METHODS: Triplicates of hESC were labeled by simple incubation with 50 μg/ml of ferumoxides before or after differentiation into CM, then imaged on a 7T MR scanner using a T2-weighted multi-echo spin-echo sequence. Viability, iron uptake and T2-relaxation times were compared between groups using t-tests. RESULTS: hESC-CM labeled before differentiation demonstrated significant MR effects, iron uptake and preserved function. hESC-CM labeled after differentiation showed no significant iron uptake or change in MR signal (P < 0.05). Morphology, differentiation and viability were consistent between experimental groups. CONCLUSION: hESC-CM should be labeled prior to CM differentiation to achieve a significant MR signal. This technique permits monitoring delivery and engraftment of hESC-CM for potential advancements of stem cell-based therapies in the reconstitution of damaged myocardium. |
| | |
Authors:
|
Rosalinda T Castaneda; Sophie Boddington; Tobias D Henning; Mike Wendland; Lydia Mandrussow; Siyuan Liu; Heike Daldrup-Link |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-5-19 |
Journal Detail:
|
Title: Pediatric radiology Volume: - ISSN: 1432-1998 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-5-19 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0365332 Medline TA: Pediatr Radiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Pediatric Radiology, Lucile Packard Children's Hospital, Stanford School of Medicine, 725 Welch Rd, Rm 1665, Stanford, CA, 94305-5654, USA, rcastan@stanford.edu. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Common and uncommon vascular rings and slings: a multi-modality review.
Next Document: MRI of closed spinal dysraphisms.