Document Detail


Labeling of fusion proteins with synthetic fluorophores in live cells.
MedLine Citation:
PMID:  15226507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A general approach for the sequential labeling of fusion proteins of O(6)-alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifically with different fluorophores, and the fluorescence labeling can be used for applications such as multicolor analysis of dynamic processes and fluorescence resonance energy transfer measurements. The facile access to a variety of different AGT substrates as well as the specificity of the labeling reaction should make the approach an important tool to study protein function in live cells.
Authors:
Antje Keppler; Horst Pick; Claudio Arrivoli; Horst Vogel; Kai Johnsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-06-28
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  101     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-08     Completed Date:  2004-07-30     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9955-9     Citation Subset:  IM    
Affiliation:
Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Calcium / metabolism
Cricetinae
Fluorescence Resonance Energy Transfer
Fluorescent Dyes*
O(6)-Methylguanine-DNA Methyltransferase / metabolism*
Recombinant Fusion Proteins / metabolism*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Recombinant Fusion Proteins; 7440-70-2/Calcium; EC 2.1.1.63/O(6)-Methylguanine-DNA Methyltransferase
Comments/Corrections

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