| Labeling of fusion proteins with synthetic fluorophores in live cells. | |
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MedLine Citation:
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PMID: 15226507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A general approach for the sequential labeling of fusion proteins of O(6)-alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifically with different fluorophores, and the fluorescence labeling can be used for applications such as multicolor analysis of dynamic processes and fluorescence resonance energy transfer measurements. The facile access to a variety of different AGT substrates as well as the specificity of the labeling reaction should make the approach an important tool to study protein function in live cells. |
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Authors:
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Antje Keppler; Horst Pick; Claudio Arrivoli; Horst Vogel; Kai Johnsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-06-28 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 101 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-07-08 Completed Date: 2004-07-30 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 9955-9 Citation Subset: IM |
Affiliation:
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Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells Calcium / metabolism Cricetinae Fluorescence Resonance Energy Transfer Fluorescent Dyes* O(6)-Methylguanine-DNA Methyltransferase / metabolism* Recombinant Fusion Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Recombinant Fusion Proteins; 7440-70-2/Calcium; EC 2.1.1.63/O(6)-Methylguanine-DNA Methyltransferase |
| Comments/Corrections | |
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