| Labeling D+ RBCs for flow cytometric quantification of fetomaternal hemorrhage after the RBCs have been coated with anti-D. | |
| | |
MedLine Citation:
|
PMID: 11493739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: D- patients may receive Rh immunoglobulin (RhIg) before blood samples are taken for estimation of the volume of fetomaternal hemorrhage (FMH) by flow cytometry. Anti-D bound to the fetal D+ cells may then block the binding of conjugated D MoAb. This may reduce the fluorescence of the D+ cells, which would lead to ambiguity over setting the positions of the markers on histograms and may result in erroneous values of FMH. STUDY DESIGN AND METHODS: Labeling methods were compared by using FITC-BRAD 3 (anti-D) and/or FITC-anti-IgG (Fab fragment) with mixtures of D+ (R1r) and D- (rr) cells when the D+ cells had first been coated with various amounts (0 molecules/cell and 600-13,000 molecules/cell) of anti-D (RhIg). Variables examined were antibody concentrations, the order and times of incubation with the antibodies, and the effect of washing between the uptake of the antibodies used. RESULTS: In all cases, D+ cells were strongly labeled after incubation with 50 microL of FITC-BRAD-3 and then after washing with 50 microL of FITC-anti-human IgG, with both incubations being for 30 minutes at 37 degrees C. With this double-staining procedure, the fluorescence of D+ cells was found to be similar regardless of how much anti-D (RhIg) was previously bound and greater than that with FITC-BRAD-3 alone, giving an enhanced signal-to-noise ratio. CONCLUSION: As the testing laboratory may not know if the patient has received prophylactic RhIg, this labeling method would be suitable for all samples. |
| | |
Authors:
|
B M Kumpel |
Related Documents
:
|
17571059 - Generation of a fluorescently labeled endogenous protein library in living human cells. 20039629 - Array-based sensing of normal, cancerous, and metastatic cells using conjugated fluores... 21249379 - Short- and long-term effects of small bowel resection: a unique histological study in a... 17635549 - A comparative study of the cytometric characteristics of high and low nucleic-acid bact... 17211879 - Flow cytometry to sort mammalian cells in cytokinesis. 17270179 - Three-dimensional cell organization leads to almost immediate hre activity as demonstra... 11985389 - T cell vaccination in multiple sclerosis: results of a preliminary study. 18008349 - Case report and review of lupus erythematosus cells in cytology fluids. 20826729 - Direct effects of retinoic acid on entry of fetal male germ cells into meiosis in mice. |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Transfusion Volume: 41 ISSN: 0041-1132 ISO Abbreviation: Transfusion Publication Date: 2001 Aug |
Date Detail:
|
Created Date: 2001-08-08 Completed Date: 2001-08-23 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
|
Languages: eng Pagination: 1059-63 Citation Subset: IM |
Affiliation:
|
International Blood Group Reference Laboratory, Southmead Road, Bristol, United Kingdom. belinda.kumpel@nbs.nhs.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Antibodies, Monoclonal / diagnostic use Erythrocytes / immunology*, metabolism Female Fetal Blood Fetomaternal Transfusion / blood, diagnosis*, immunology Flow Cytometry / methods*, standards Humans Isoantibodies / analysis, metabolism Pregnancy Rh-Hr Blood-Group System / immunology* Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Isoantibodies; 0/Rh-Hr Blood-Group System; 0/Rho(D) antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A D(V)-like phenotype is obliterated by A226P in the partial D DBS.
Next Document: Effects of colloidal resuscitation fluids on the neutrophil respiratory burst.