Document Detail


Labeled avidin-biotin enzyme-linked immunosorbent assay for detecting antibody to infectious laryngotracheitis virus in chickens.
MedLine Citation:
PMID:  2838015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A labeled avidin-biotin enzyme-linked immunosorbent assay (LAB-ELISA) for detecting antibody to infectious laryngotracheitis (ILT) virus in chicken sera was developed and compared with ordinary ELISA. Purified ILT virus, biotin-labeled anti-chicken IgG rabbit IgG conjugate, and horseradish-peroxidase-labeled avidin were used in the LAB-ELISA. When sera from farm chickens were tested by serum neutralization (SN) and two kinds of ELISA, the correlation rate between SN and LAB-ELISA was 50/50 (100%), and that between SN and ordinary ELISA was 39/50 (78%). In LAB-ELISA, all of the sera that were antibody-negative by SN had low absorbance (A) values (below 0.05), and the A values were closely correlated with the SN indexes. In ordinary ELISA, however, the sera antibody-negative by SN had various A values ranging from 0.06 to 0.32. LAB-ELISA had much lower nonspecific reactions than ordinary ELISA against sera from ILT-negative chickens, even when chickens were 30 weeks old. ILT antibody production after ILT vaccination could be detected by LAB-ELISA. A values peaked 5 weeks postinoculation and were maintained for 17 weeks.
Authors:
Y Ohkubo; K Shibata; T Mimura; I Takashima
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Avian diseases     Volume:  32     ISSN:  0005-2086     ISO Abbreviation:  Avian Dis.     Publication Date:    1988 Jan-Mar
Date Detail:
Created Date:  1988-07-27     Completed Date:  1988-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370617     Medline TA:  Avian Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  24-31     Citation Subset:  IM    
Affiliation:
Department of Animal Pathology, Nisshin Flour Milling Co. Ltd., Saitama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Viral / analysis*
Avidin / diagnostic use*
Biotin / diagnostic use*
Chickens / immunology*
Enzyme-Linked Immunosorbent Assay
Herpesviridae / immunology*
Herpesvirus 1, Gallid / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 1405-69-2/Avidin; 58-85-5/Biotin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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