| Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy. | |
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MedLine Citation:
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PMID: 20615007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process. |
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Authors:
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Wen Lo; Tsung-Jen Wang; Wei-Liang Chen; Chiu-Mei Hsueh; Shean-Jen Chen; Yang-Fang Chen; Hsiu-Chu Chou; Pi-Jung Lin; Fung-Rong Hu; Chen-Yuan Dong |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical optics Volume: 15 ISSN: 1560-2281 ISO Abbreviation: J Biomed Opt Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-07-09 Completed Date: 2010-10-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: United States |
Other Details:
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Languages: eng Pagination: 036005 Citation Subset: IM |
Affiliation:
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National Taiwan University, Department of Physics and Center of Quantum Science and Engineering, Taipei 106, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Proliferation / drug effects Cornea / cytology, pathology, physiology Fibroblasts Image Processing, Computer-Assisted Immunohistochemistry Linear Models Microscopy, Acoustic / methods* Microscopy, Fluorescence, Multiphoton / methods* Mitomycin / pharmacology* Photorefractive Keratectomy* Rabbits Wound Healing / drug effects* |
| Chemical | |
Reg. No./Substance:
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50-07-7/Mitomycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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