Document Detail


Label-free luminescent oligonucleotide-based probes.
MedLine Citation:
PMID:  23348604     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Breakthrough advances in chemistry and biology over the last two decades have vastly expanded the repertoire of nucleic acid structure and function with potential application in multiple areas of science and technology, including sensing and analytical applications. DNA oligonucleotides represent popular tools for the development of sensing platforms due to their low cost, rich structural polymorphism, and their ability to bind to cognate ligands with sensitivity and specificity rivaling those for protein enzymes and antibodies. In this review, we give an overview of the "label-free" approach that has been a particular focus of our group and others for the construction of luminescent DNA-based sensing platforms. The label-free strategy aims to overcome some of the drawbacks associated with the use of covalently-labeled oligonucleotides prevalent in electrochemical and optical platforms. Label-free DNA-based probes harness the selective interaction between luminescent dyes and functional oligonucleotides that exhibit a "structure-switching" response upon binding to analytes. Based on the numerous examples of label-free luminescent DNA-based probes reported recently, we envisage that this field would continue to thrive and mature in the years to come.
Authors:
Dik-Lung Ma; Hong-Zhang He; Ka-Ho Leung; Hai-Jing Zhong; Daniel Shiu-Hin Chan; Chung-Hang Leung
Related Documents :
6087274 - Alteration of apparent restriction endonuclease recognition specificities by dna methyl...
15608184 - Rebase--restriction enzymes and dna methyltransferases.
1892954 - Isolation of restrictible dna.
9199404 - Mutations in the res subunit of the ecopi restriction enzyme that affect atp-dependent ...
21170394 - Micropatterning of aptamer beacons to create cytokine-sensing surfaces.
19171784 - Allosteric control of escherichia coli rrna promoter complexes by dksa.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-25
Journal Detail:
Title:  Chemical Society reviews     Volume:  -     ISSN:  1460-4744     ISO Abbreviation:  Chem Soc Rev     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0335405     Medline TA:  Chem Soc Rev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. edmondma@hkbu.edu.hk.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Patients' perspective of wide-awake hand surgery -- 100 consecutive cases.
Next Document:  Head and Neck Injuries in Professional Soccer.