| Label-free live-cell imaging with confocal Raman microscopy. | |
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MedLine Citation:
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PMID: 22339873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Confocal Raman spectroscopy is a noninvasive alternative to established cell imaging methods because it does not require chemical fixation, the use of fluorescent markers, or genetic engineering. In particular, single live-cell, high-resolution imaging by confocal Raman microscopy is desirable because it allows further experiments concerning the individually investigated cells. However, to derive meaningful images from the spectroscopic data, one must identify cell components within the dataset. Using immunofluorescence images as a reference, we derive Raman spectral signatures by means of information measures to identify cell components such as the nucleus, the endoplasmic reticulum, the Golgi apparatus, and mitochondria. The extracted signatures allow us to generate representations equivalent to conventional (immuno)fluorescence images with more than three cell components at a time, exploiting the Raman spectral information alone. |
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Authors:
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Katharina Klein; Alexander M Gigler; Thomas Aschenbrenner; Roberto Monetti; Wolfram Bunk; Ferdinand Jamitzky; Gregor Morfill; Robert W Stark; Jürgen Schlegel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biophysical journal Volume: 102 ISSN: 1542-0086 ISO Abbreviation: Biophys. J. Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-02-20 Completed Date: 2012-06-07 Revised Date: 2013-04-05 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 360-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Division of Neuropathology, Institute of Pathology, Technische Universität München, Munich, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Cell Survival Humans Microscopy, Confocal / methods* Microscopy, Fluorescence Spectrum Analysis, Raman / methods* |
| Comments/Corrections | |
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