Document Detail

Label-free imaging, detection, and mass measurement of single viruses by surface plasmon resonance.
MedLine Citation:
PMID:  20798340     Owner:  NLM     Status:  MEDLINE    
We report on label-free imaging, detection, and mass/size measurement of single viral particles in solution by high-resolution surface plasmon resonance microscopy. Diffraction of propagating plasmon waves along a metal surface by the viral particles creates images of the individual particles, which allow us to detect the binding of the viral particles to surfaces functionalized with and without antibodies. We show that the intensity of the particle image is related to the mass of the particle, from which we determine the mass and mass distribution of influenza viral particles with a mass detection limit of approximately 1 ag (or 0.2 fg/mm(2)). This work demonstrates a multiplexed method to measure the masses of individual viral particles and to study the binding activity of the viral particles.
Shaopeng Wang; Xiaonan Shan; Urmez Patel; Xinping Huang; Jin Lu; Jinghong Li; Nongjian Tao
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-08-26
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  107     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-15     Completed Date:  2010-10-28     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  16028-32     Citation Subset:  IM    
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MeSH Terms
Influenza A Virus, H1N1 Subtype / chemistry*,  isolation & purification
Nanoparticles / chemistry
Silicon Dioxide / chemistry
Surface Plasmon Resonance / instrumentation,  methods*
Surface Properties
Virion / chemistry
Grant Support
Reg. No./Substance:
7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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