Document Detail


Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes.
MedLine Citation:
PMID:  23329132     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.
Authors:
Yadveer S Grewal; Muhammad J A Shiddiky; Sean A Gray; Kris M Weigel; Gerard A Cangelosi; Matt Trau
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemical communications (Cambridge, England)     Volume:  49     ISSN:  1364-548X     ISO Abbreviation:  Chem. Commun. (Camb.)     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-24     Completed Date:  2013-08-14     Revised Date:  2014-02-23    
Medline Journal Info:
Nlm Unique ID:  9610838     Medline TA:  Chem Commun (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1551-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigens, Protozoan / analysis*,  immunology
Electrochemical Techniques*
Electrodes
Electron Transport
Entamoeba histolytica / metabolism*
Ferricyanides / chemistry
Gold / chemistry
Humans
Immunoassay
Recombinant Proteins / biosynthesis,  genetics,  immunology
Saccharomyces cerevisiae / metabolism*
Single-Chain Antibodies / genetics,  immunology*,  metabolism
Grant Support
ID/Acronym/Agency:
U01 AI082186/AI/NIAID NIH HHS; U01AI082186-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Protozoan; 0/Ferricyanides; 0/Recombinant Proteins; 0/Single-Chain Antibodies; 13408-62-3/hexacyanoferrate III; 7440-57-5/Gold
Comments/Corrections

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