| Label-free detection of multiple bacterial pathogens using light-scattering sensor. | |
| | |
MedLine Citation:
|
PMID: 18945607 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Technologies for rapid detection and classification of bacterial pathogens are crucial for securing the food supply. This report describes a light-scattering sensor capable of real-time detection and identification of colonies of multiple pathogens without the need for a labeling reagent or biochemical processing. Bacterial colonies consisting of the progeny of a single parent cell scatter light at 635 nm to produce unique forward-scatter signatures. Zernike moment invariants and Haralick descriptors aid in feature extraction and construction of the scatter-signature image library. The method is able to distinguish bacterial cultures at the genus and species level for Listeria, Staphylococcus, Salmonella, Vibrio, and Escherichia with an accuracy of 90-99% for samples derived from food or experimentally infected animal. Varied amounts of exopolysaccharide produced by the bacteria causes changes in phase modulation distributions, resulting in strikingly different scatter signatures. With the aid of a robust database the method can potentially detect and identify any bacteria colony essentially instantaneously. Unlike other methods, it does not destroy the sample, but leaves it intact for other confirmatory testing, if needed, for forensic or outbreak investigations. |
| | |
Authors:
|
Padmapriya P Banada; Karleigh Huff; Euiwon Bae; Bartek Rajwa; Amornrat Aroonnual; Bulent Bayraktar; Abrar Adil; J Paul Robinson; E Daniel Hirleman; Arun K Bhunia |
Related Documents
:
|
19610347 - Real-time pcr as a tool for detection of pathogenic bacteria on contaminated food conta... 10423737 - Detection of salmonella spp. in food by a rapid pcr-hybridization procedure. 11943377 - Detection of genetically modified organisms in foods. 16640287 - Roche/biotecon diagnostics lightcycler foodproof l. monocytogenes detection kit in comb... 2398997 - Scanning electron microscopy of corneal incisions using steel, diamond, and sapphire bl... 17294697 - Diosgenin contents and dna fingerprint screening of various yam (dioscorea sp.) genotypes. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-11 |
Journal Detail:
|
Title: Biosensors & bioelectronics Volume: 24 ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2009 Feb |
Date Detail:
|
Created Date: 2009-02-02 Completed Date: 2009-05-26 Revised Date: 2009-07-14 |
Medline Journal Info:
|
Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
|
Languages: eng Pagination: 1685-92 Citation Subset: IM |
Affiliation:
|
Department of Food Science, Molecular Food Microbiology Laboratory, Purdue University, IN 47907, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biosensing Techniques
/
instrumentation* Colony Count, Microbial / instrumentation* Equipment Design Equipment Failure Analysis Light Refractometry / instrumentation* Reproducibility of Results Scattering, Radiation Sensitivity and Specificity Staining and Labeling |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Heroin in brown, black and white: structural factors and medical consequences in the US heroin marke...
Next Document: Development of disposable lipid biosensor for the determination of total cholesterol.