| Label-free and amplified electrochemical detection of cytokine based on hairpin aptamer and catalytic DNAzyme. | |
| | |
MedLine Citation:
|
PMID: 22193340 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
In this work, by incorporating a specific DNAzyme sequence into a hairpin aptamer probe, we describe a label-free and sensitive method for electrochemical detection of cytokines using recombinant human IFN-γ as the model analyte. The hairpin aptamer probes are immobilized on a gold electrode through self-assembly. The presence of IFN-γ opens the hairpin structure and forms the hemin/G-quadruplex peroxidase-mimicking DNAzyme with subsequent addition of hemin. The peroxidase-mimicking DNAzyme catalyzes the electro-reduction of H(2)O(2) and amplifies the current response for IFN-γ detection, which enables the monitoring of IFN-γ at the sub-nanomolar level. The proposed sensor also shows high selectivity towards the target analyte. Our strategy thus opens new opportunities for label-free and amplified detection of different types of cytokines. |
| | |
Authors:
|
Haixia Zhang; Bingying Jiang; Yun Xiang; Yaqin Chai; Ruo Yuan |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-23 |
Journal Detail:
|
Title: The Analyst Volume: - ISSN: 1364-5528 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372652 Medline TA: Analyst Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China. yunatswu@swu.edu.cn yuanruo@swu.edu.cn. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expression of Brassica oleracea FtsZ1-1 and MinD alters chloropl...
Next Document: Changes in strength and power qualities over two years in volleyball players transitioning from juni...