Document Detail


Label-free and Ultrasensitive Electrochemical Detection of Nucleic Acids Based on Autocatalytic and Exonuclease III-assisted Target Recycling Strategy.
MedLine Citation:
PMID:  23320625     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In this work, a very simple, label-free, isothermal and ultrasensitive electrochemical DNA biosensor has been developed based on an autocatalytic and exonuclease III (Exo III)-assisted target recycling amplification strategy. A duplex DNA probe constructed by the hybridization of a quadruplex-forming oligomer with a molecular beacon is ingeniously designed and assembled on the electrode as recognition element. Upon sensing of the analyte nucleic acid, the strand of molecular beacon in the duplex DNA probe could be stepwise removed by Exo III accompanied by the releasing of target DNA and autonomous generation of new secondary target DNA fragment for the successive hybridization and cleavage process. Simultaneously, numerous quadruplex-forming oligomers are liberated and folded into G-quadruplex-hemin complexes with the help of K+ and hemin on the electrode surface to give a remarkable electrochemical response. Because of this autocatalytic target recycling amplification and the specifically catalyzed formation of G-quadruplex-hemin complexes, this newly designed protocol provides an ultrasensitive electrochemical detection of DNA down to the 10 fM level and could discriminate mismatched DNA from perfectly matched target DNA, and holds a great potential for early diagnosis in gene-related diseases. It further could be developed as a universal protocol for the detection of various DNA sequences and may be extended for the detection of aptamer-binding molecules.
Authors:
Shufeng Liu; Chunfeng Wang; Chengxin Zhang; Ying Wang; Bo Tang
Related Documents :
23799235 - On the nature of dna hyperchromic effect.
24089735 - Statistical physics of self-replication.
15988005 - A conserved swi2/snf2 atpase motif couples atp hydrolysis to chromatin remodeling.
12634355 - Hepatitis c virus ns3 atpases/helicases from different genotypes exhibit variations in ...
8000105 - A melas (mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episo...
11100865 - Mobility and activation energy of single-stranded dna in denaturing cross-linked polyac...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  People with dementia and the hospital environment: the view of patients and family carers.
Next Document:  Filtration of sea louse, Lepeophtheirus salmonis, copepodids by the blue mussel, Mytilus edulis, and...