| Label-free Raman observation of cytochrome c dynamics during apoptosis. | |
| | |
MedLine Citation:
|
PMID: 22184220 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
We performed label-free observation of molecular dynamics in apoptotic cells by Raman microscopy. Dynamic changes in cytochrome c distribution at the Raman band of 750 cm(-1) were observed after adding an apoptosis inducer to the cells. The comparison of mitochondria fluorescence images and Raman images of cytochrome c confirmed that changes in cytochrome c distribution can be distinguished as release of cytochrome c from mitochondria. Our observation also revealed that the redox state of cytochrome c was maintained during the release from the mitochondria. Monitoring mitochondrial membrane potential with JC-1 dye confirmed that the observed cytochrome c release was associated with apoptosis. |
| | |
Authors:
|
Masaya Okada; Nicholas Isaac Smith; Almar Flotildes Palonpon; Hiromi Endo; Satoshi Kawata; Mikiko Sodeoka; Katsumasa Fujita |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-19 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: - ISSN: 1091-6490 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Exploring one-state downhill protein folding in single molecules.
Next Document: Constraints on continental crustal mass loss via chemical weathering using lithium and its isotopes.