| Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy. | |
| | |
MedLine Citation:
|
PMID: 23062077 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Abstract We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screening compatible application, we automatized a digital holographic microscope for image acquisition of cells using commercially available 96-well plates. Data generated through both label-free DHM imaging and fluorescence-based methods were in good agreement for cell viability identification and a Z'-factor close to 0.9 was determined, validating the robustness of DHM assay for phenotypic screening. Further, an excellent correlation was obtained between experimental cytotoxicity dose-response curves and known IC(50) values for different toxic compounds. For comparable results, DHM has the major advantages of being label free and close to an order of magnitude faster than automated standard fluorescence microscopy. |
| | |
Authors:
|
Jonas Kühn; Etienne Shaffer; Julien Mena; Billy Breton; Jérôme Parent; Benjamin Rappaz; Marc Chambon; Yves Emery; Pierre Magistretti; Christian Depeursinge; Pierre Marquet; Gerardo Turcatti |
Related Documents
:
|
23026997 - Live-cell migration and adhesion turnover assays. 21893407 - Quantitative microscopy and imaging tools for the mechanical analysis of morphogenesis. 22183727 - A novel method for spatially complex diffraction-limited photoactivation and photobleac... 23640257 - Molecular imaging and tracking stem cells in neurosciences. 22009867 - An mri-compatible robotic system with hybrid tracking for mri-guided prostate intervent... 20445137 - Computed tomography shielding methods: a literature review. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-12 |
Journal Detail:
|
Title: Assay and drug development technologies Volume: - ISSN: 1557-8127 ISO Abbreviation: Assay Drug Dev Technol Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-15 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101151468 Medline TA: Assay Drug Dev Technol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
1 Biomolecular Screening Facility (BSF), Swiss Ecole Polytechnique Fédérale de Lausanne (EPFL) , Lausanne, Switzerland . |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A fresh look at paralytics in the critically ill: real promise and real concern.
Next Document: Development and Validation of a Single-Well Cell-Based Assay for the Detection of Endogenous Phospho...