Document Detail


Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy.
MedLine Citation:
PMID:  23062077     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screening compatible application, we automatized a digital holographic microscope for image acquisition of cells using commercially available 96-well plates. Data generated through both label-free DHM imaging and fluorescence-based methods were in good agreement for cell viability identification and a Z'-factor close to 0.9 was determined, validating the robustness of DHM assay for phenotypic screening. Further, an excellent correlation was obtained between experimental cytotoxicity dose-response curves and known IC(50) values for different toxic compounds. For comparable results, DHM has the major advantages of being label free and close to an order of magnitude faster than automated standard fluorescence microscopy.
Authors:
Jonas Kühn; Etienne Shaffer; Julien Mena; Billy Breton; Jérôme Parent; Benjamin Rappaz; Marc Chambon; Yves Emery; Pierre Magistretti; Christian Depeursinge; Pierre Marquet; Gerardo Turcatti
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-12
Journal Detail:
Title:  Assay and drug development technologies     Volume:  -     ISSN:  1557-8127     ISO Abbreviation:  Assay Drug Dev Technol     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101151468     Medline TA:  Assay Drug Dev Technol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1 Biomolecular Screening Facility (BSF), Swiss Ecole Polytechnique Fédérale de Lausanne (EPFL) , Lausanne, Switzerland .
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