| Label-Free Cell Separation Using a Tunable Magnetophoretic Repulsion Force. | |
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MedLine Citation:
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PMID: 22380761 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This paper describes a new label-free cell separation method using a magnetic repulsion force resulting from the magnetic susceptibility difference between cells and a paramagnetic buffer solution in a microchannel. The difference in the magnetic forces acting on different-sized cells is enhanced by adjusting the magnetic susceptibility of the surrounding medium, which depends on the concentration of paramagnetic salts, such as biocompatible gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA), dissolved therein. As proof-of-concept demonstration, Gd-DTPA solutions at concentrations of 0 to 80 mM were applied to separate U937 cells from red blood cells (RBCs) and to distinguish two different-sized polystyrene (PS) beads (8 and 10 μm in diameter). By increasing the Gd-DTPA concentration from 0 to 40 mM, the separation resolution of PS beads was increased from 0.08 to 0.91. Additionally, we successfully achieved label-free separation of U937 cells from RBCs with >90% purity and 1 × 10<sup>5</sup> cells/h throughput using a 40 mM Gd-DTPA solution. |
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Authors:
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Fengshan Shen; Hyundoo Hwang; Young Ki Hahn; Je-Kyun Park |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-29 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-3-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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