Document Detail


Label-Free Cell Separation Using a Tunable Magnetophoretic Repulsion Force.
MedLine Citation:
PMID:  22380761     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This paper describes a new label-free cell separation method using a magnetic repulsion force resulting from the magnetic susceptibility difference between cells and a paramagnetic buffer solution in a microchannel. The difference in the magnetic forces acting on different-sized cells is enhanced by adjusting the magnetic susceptibility of the surrounding medium, which depends on the concentration of paramagnetic salts, such as biocompatible gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA), dissolved therein. As proof-of-concept demonstration, Gd-DTPA solutions at concentrations of 0 to 80 mM were applied to separate U937 cells from red blood cells (RBCs) and to distinguish two different-sized polystyrene (PS) beads (8 and 10 μm in diameter). By increasing the Gd-DTPA concentration from 0 to 40 mM, the separation resolution of PS beads was increased from 0.08 to 0.91. Additionally, we successfully achieved label-free separation of U937 cells from RBCs with >90% purity and 1 × 10<sup>5</sup> cells/h throughput using a 40 mM Gd-DTPA solution.
Authors:
Fengshan Shen; Hyundoo Hwang; Young Ki Hahn; Je-Kyun Park
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-29
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-3-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  FINITE ELEMENT ANALYSIS OF PROVISIONAL STRUCTURES OF IMPLANT-SUPPORTED COMPLETE PROSTHESES.
Next Document:  Assessment of Echocardiographic Left Atrial Size: Accuracy of M-Mode and Two-Dimensional Methods and...