| LYCAT, a homologue of C. elegans acl-8, acl-9 and acl-10, determines the fatty acid composition of phosphatidylinositol in mice. | |
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MedLine Citation:
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PMID: 22172515 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Mammalian phosphatidylinositol (PI) has a unique fatty acid composition in that 1-stearoyl-2-arachidonoyl species is predominant. This fatty acid composition is formed through fatty acid remodeling by sequential deacylation and reacylation. We recently identified three C. elegans acyltransferases (ACL-8, ACL-9 and ACL-10) that incorporate stearic acid into the sn-1 position of PI. Mammalian LYCAT, which is the closest homologue of ACL-8, ACL-9 and ACL-10, was originally identified as a lysocardiolipin acyltransferase by an in vitro assay and was subsequently reported to possess acyltransferase activity towards various anionic lysophospholipids. However, the in vivo role of mammalian LYCAT in phospholipid fatty acid metabolism has not been well elucidated. In this study, we generated LYCAT-deficient mice and demonstrated that LYCAT determined the fatty acid composition of PI in vivo. LYCAT-deficient mice were outwardly healthy and fertile. In the mice, stearoyl-CoA acyltransferase activity towards the sn-1 position of PI was reduced, and the fatty acid composition of PI, but not those of other major phospholipids was altered. Furthermore, expression of mouse LYCAT rescued the phenotype of C. elegans acl-8 acl-9 acl-10 triple mutants. Our data indicate that LYCAT is a determinant of PI molecular species and its function is conserved in C. elegans and mammals. |
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Authors:
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Rieko Imae; Takao Inoue; Yasuko Nakasaki; Yasunori Uchida; Yosuke Ohba; Nozomu Kono; Hiroki Nakanishi; Takehiko Sasaki; Shohei Mitani; Hiroyuki Arai |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-14 |
Journal Detail:
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Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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