| LRRK2 expression in normal and pathologic human brain and in human cell lines. | |
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MedLine Citation:
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PMID: 17021400 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mutations in the leucine-rich repeat kinase 2 gene (LRRK2) have been recently identified in families with autosomal-dominant late-onset Parkinson disease. We report that by reverse transcriptase-polymerase chain reaction, the mRNA of LRRK2 is expressed in soluble extracts of human brain, liver, and heart and in cultured human astrocytes, microglia, and oligodendroglia as well as in human neuroblastoma cell lines. We find by Western blotting using a polyclonal antibody of the leucine-rich repeat kinase 2 protein (Lrrk2) specific for C-terminal residues 2,511-2,527 that an apparent full-length protein and several of its fractions are expressed in soluble extracts of normal human brain. By immunocytochemistry, the antibody recognizes neurons, and more weakly astrocytes and microglia, in normal brain tissue. It intensely labels Lewy bodies in Parkinson disease and related neurodegenerative disorders. It also labels a subset of neurofibrillary tangles in Alzheimer disease and the Parkinsonism dementia complex of Guam (PDCG). It labels thorn-shaped astrocytes and oligodendroglial coiled bodies in PDCG; oligodendroglial inclusions in multiple system atrophy; Pick bodies in Pick disease; nuclear and cytoplasmic inclusions in Huntington disease; and intraneuronal and glial inclusions in amyotrophic lateral sclerosis. In summary, LRRK2 is constitutively expressed in neurons and also in glial cells of human brain. It strongly associates with pathological inclusions in several neurodegenerative disorders. |
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Authors:
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Judith Miklossy; Tetsuaki Arai; Jian-Ping Guo; Andis Klegeris; Sheng Yu; Edith G McGeer; Patrick L McGeer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neuropathology and experimental neurology Volume: 65 ISSN: 0022-3069 ISO Abbreviation: J. Neuropathol. Exp. Neurol. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-05 Completed Date: 2006-11-14 Revised Date: 2012-06-25 |
Medline Journal Info:
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Nlm Unique ID: 2985192R Medline TA: J Neuropathol Exp Neurol Country: United States |
Other Details:
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Languages: eng Pagination: 953-63 Citation Subset: IM |
Affiliation:
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Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, BC, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Astrocytes / metabolism Biological Markers / analysis* Blotting, Western Brain / metabolism*, pathology Cells, Cultured Female Humans Immunohistochemistry Liver / metabolism Male Microglia / metabolism Middle Aged Myocardium / metabolism Neurodegenerative Diseases / metabolism*, pathology Oligodendroglia / metabolism Protein-Serine-Threonine Kinases / biosynthesis* RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/RNA, Messenger; EC 2.7.11.1/LRRK2 protein, human; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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