| LPS-induced inflammation downregulates mammary gland glucose, fatty acid, and L-carnitine transporter expression at different lactation stages. | |
| | |
MedLine Citation:
|
PMID: 20381822 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Glucose, fatty acids, and L-carnitine are important substrates that support mammary epithelial cell metabolism, biosynthetic capacity, and milk yield and composition. Our study investigated the effects of LPS-induced inflammation on the expression of several glucose, fatty acid, and L-carnitine transporters in the lactating rat mammary gland at different lactation stages. Day 4, 11, and 18 lactating rats (n=3/treatment) were administered LPS (1 mg/kg) or saline by intraperitoneal (i.p.) injection. Fold differences in the mRNA expression of glucose transporters Glut1, Glut8 and Sglt1, fatty acid transporters Fatp1, Fatp4 and Fabp3, and L-carnitine transporters Octn1, Octn2, and Octn3 were determined using the Comparative C(T) method. The mRNA expression levels of all transporters evaluated, except Fatp4 and Octn2 were markedly higher in mammary gland at lactation day 11 compared to lactation day 4. LPS caused a marked decrease in transporter mRNA expression at each lactation stage except for Octn3 and Fatp1, which were markedly increased with LPS administration at lactation day 4, and Sglt1, which was slightly increased at day 11 of lactation. Our results suggest LPS-induced inflammation generally downregulates glucose, fatty acid, and L-carnitine transporter expression. Whether such changes lead to reductions in transporter substrate availability to the lactating mammary epithelial cell requires investigation since decreases in the availability of these nutrients may significantly impact mammary epithelial function and milk quality and yield. |
| | |
Authors:
|
Binbing Ling; Jane Alcorn |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-09 |
Journal Detail:
|
Title: Research in veterinary science Volume: 89 ISSN: 1532-2661 ISO Abbreviation: Res. Vet. Sci. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-08-24 Completed Date: 2011-01-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0401300 Medline TA: Res Vet Sci Country: England |
Other Details:
|
Languages: eng Pagination: 200-2 Citation Subset: IM |
Copyright Information:
|
2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
College of Pharmacy and Nutrition, University of Saskatchewan, 110 Science Place, Saskatoon, Saskatchewan, Canada S7N5C9. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Down-Regulation Fatty Acids / metabolism Female Gene Expression Regulation / physiology Glucose / metabolism Inflammation / chemically induced* Lactation / physiology* Lipopolysaccharides / toxicity* Mammary Glands, Animal / metabolism* Mastitis / chemically induced*, metabolism Organic Cation Transport Proteins / genetics, metabolism RNA, Messenger / genetics, metabolism Rats |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids; 0/Lipopolysaccharides; 0/Organic Cation Transport Proteins; 0/RNA, Messenger; 0/Slc22a5 protein, rat; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Fatty acid derivatives and dammarane triterpenes from the glandular trichome exudates of Ibicella lu...
Next Document: 5-Aminolaevulinic acid enhances ultrasound-induced mitochondrial damage in K562 cells.