Document Detail


LOCATE enzyme-linked immunosorbent assay for detection of Salmonella in food: collaborative study.
MedLine Citation:
PMID:  9549076     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A collaborative study was performed in 27 laboratories to validate the enzyme-linked immunosorbent procedure LOCATE for rapid detection of Salmonella in foods. Results were read visually and with a microtiter plate reader. The LOCATE method was compared with the Bacteriological Analytical Manual (BAM)/AOAC INTERNATIONAL culture method for detecting Salmonella in 6 foods: milk chocolate, nonfat dry milk, dried whole egg, soy flour, ground black pepper, and ground raw turkey. Two foods--dried whole egg and black pepper--required repeat rounds because insufficient data sets were produced initially (AOAC INTERNATIONAL stipulates a minimum of 15 sets per food type). Each laboratory tested one or more of the 6 foods. A total of 1 439 samples were analyzed, and no significant differences (P < 0.05) were observed between LOCATE with either visual or reader detection and BAM/AOAC INTERNATIONAL results. The LOCATE screening method with visual or reader detection is recommended for Official First Action Approval.
Authors:
V Gangar; M S Curiale; A D'Onorio; C Donnelly; P Dunnigan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  81     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    1998 Mar-Apr
Date Detail:
Created Date:  1998-05-05     Completed Date:  1998-05-05     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  419-37     Citation Subset:  IM    
Affiliation:
Silliker Laboratories Group, Inc., Corporate Research Center, South Holland, IL 60473, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cacao / chemistry,  microbiology
Eggs / analysis,  microbiology
Enzyme-Linked Immunosorbent Assay
Flour / analysis,  microbiology
Food Contamination / analysis*
Food Microbiology
Indicators and Reagents
Meat / analysis,  microbiology
Milk / chemistry,  microbiology
Reproducibility of Results
Salmonella / chemistry*
Serotyping
Spices / analysis,  microbiology
Turkeys
Chemical
Reg. No./Substance:
0/Indicators and Reagents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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