Document Detail

LKB1 regulates development and the stress response in Dictyostelium.
MedLine Citation:
PMID:  22020250     Owner:  NLM     Status:  MEDLINE    
The serine/threonine kinase LKB1 is a master kinase that regulates a number of critical events such as cell transformation, polarization, development, stress response, and energy metabolism in metazoa. After multiple unsuccessful attempts of generating Dictyostelium lkb1-null cells, an RNAi-based knockdown approach proved effective. Depletion of lkb1 with a knockdown construct displayed severe reduction in prespore cell differentiation and precocious induction of prestalk cells, which were reminiscent of cells lacking GSK3. Similar to gsk3(-) cells, lkb1 depleted cells displayed lower GSK3 activity than wild type cells during development and compromised cAMP-mediated inhibition of the DIF-1 mediated ecmB induction. In response to stress insult, the kinase activity of LKB1, but not that of GSK3, increased. Therefore, LKB1 positively functions at the upstream of GSK3 during development and responds to stress insults independently from GSK3.
Sudhakar Veeranki; Seon-Hee Hwang; Tong Sun; Bohye Kim; Leung Kim
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-10-12
Journal Detail:
Title:  Developmental biology     Volume:  360     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-15     Completed Date:  2012-01-23     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  351-7     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Inc.
Department of Biological Sciences, Florida International University, Miami, FL 33199, USA.
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MeSH Terms
Amino Acid Sequence
Cell Differentiation
Cyclic AMP / metabolism
Dictyostelium / cytology,  genetics*,  metabolism
Gene Expression Regulation
Gene Knockdown Techniques
Glycogen Synthase Kinase 3 / genetics,  metabolism
Molecular Sequence Data
Protein-Serine-Threonine Kinases / genetics*,  metabolism
Protozoan Proteins / genetics*,  metabolism
RNA Interference
Stress, Physiological
Grant Support
SC1 CA143958-01A1/CA/NCI NIH HHS; SC1 CA143958-02/CA/NCI NIH HHS; SC1CA143958/CA/NCI NIH HHS
Reg. No./Substance:
0/Protozoan Proteins; 60-92-4/Cyclic AMP; EC Kinases; EC Synthase Kinase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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