| LIS1 and platelet-activating factor acetylhydrolase (Ib) catalytic subunits, expression in the mouse oocyte and zygote. | |
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MedLine Citation:
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PMID: 10371146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Platelet-activating factor is a phospholipid with several documented roles in the pre-implantation embryo. Enzymes that belong to the platelet-activating factor acetylhydrolases family inactivate platelet-activating factor. Cytosolic platelet-activating factor acetylhydrolase (Ib) is a heterotetramer composed of two catalytic subunits (alpha1/alpha2) and two regulatory LIS1 subunits. The expression of these components was monitored in the mouse oocytes and zygotes using reverse-transcribed PCR and Western blot analysis. Interestingly, these proteins are expressed in the oocyte and zygote and their expression increases after fertilization, probably due to stabilization of maternal RNA. Lis1 mRNA transcription also increases after fertilization. However, assaying for expression of a specific paternal LIS1 isoform detected no zygotic translation in the one cell stage. These findings suggest a potential role for platelet-activating factor acetylhydrolase (Ib) components in the early mouse embryo. |
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Authors:
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A Cahana; O Reiner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 451 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1999 May |
Date Detail:
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Created Date: 1999-07-06 Completed Date: 1999-07-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 99-102 Citation Subset: IM |
Affiliation:
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Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Alkyl-2-acetylglycerophosphocholine Esterase Animals Brain / metabolism Female Gene Expression Regulation, Developmental* Male Mice Mice, Knockout Microtubule-Associated Proteins* Oocytes / metabolism* Phospholipases A / metabolism* Platelet Activating Factor / metabolism* Proteins / metabolism* Tissue Distribution Zygote / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Microtubule-Associated Proteins; 0/Platelet Activating Factor; 0/Proteins; EC 3.1.1.-/Phospholipases A; EC 3.1.1.47/1-Alkyl-2-acetylglycerophosphocholine Esterase; EC 3.1.1.47/Pafah1b1 protein, mouse |
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