Document Detail


LIPID arrays: new tools in the understanding of membrane dynamics and lipid signaling.
MedLine Citation:
PMID:  15087482     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phospholipids are the structural building blocks of the membrane bilayer, which retains and regulates intra-cellular content. In addition to creating a protective barrier around the cell, lipids modulate membrane trafficking and are themselves precursors of important intracellular signaling molecules. Identification and quantification of these molecular species is essential for a more complete understanding of cell signaling pathways, and more reliable and sensitive methods are needed for determining membrane phospholipid content. Recent improvements in electrospray ionization mass spectrometry have made possible the direct identification of more than 400 phospholipid species from biological extracts of a single cell type. Changes in the cellular concentration of diverse lipids can be determined by analysis of the mass spectra by statistical algorithms. In the future, lipid arrays will be integrated with other high-throughput profiling technologies, and computational lipidomics will expand our understanding of the molecular basis of cellular processes and diseases.
Authors:
Pavlina T Ivanova; Stephen B Milne; Jeffrey S Forrester; H Alex Brown
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review    
Journal Detail:
Title:  Molecular interventions     Volume:  4     ISSN:  1543-2548     ISO Abbreviation:  Mol. Interv.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-16     Completed Date:  2008-03-24     Revised Date:  2009-06-22    
Medline Journal Info:
Nlm Unique ID:  101093789     Medline TA:  Mol Interv     Country:  United States    
Other Details:
Languages:  eng     Pagination:  86-96     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and the Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Degranulation
Cell Membrane / chemistry,  metabolism*
Mast Cells / metabolism
Microarray Analysis* / instrumentation,  methods
Molecular Structure
Phosphatidylinositol Phosphates / metabolism
Phospholipids / chemistry,  metabolism*
Signal Transduction / physiology*
Spectrometry, Mass, Electrospray Ionization
Grant Support
ID/Acronym/Agency:
GM58516/GM/NIGMS NIH HHS; NIGMS62114//PHS HHS
Chemical
Reg. No./Substance:
0/Phosphatidylinositol Phosphates; 0/Phospholipids

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