Document Detail

The LIM domain homeobox gene isl-1: conservation of human, hamster, and rat complementary deoxyribonucleic acid sequences and expression in cell types of nonneuroendocrine lineage.
MedLine Citation:
PMID:  7907017     Owner:  NLM     Status:  MEDLINE    
The LIM domain homeobox gene isl-1 was originally isolated by virtue of its ability to bind DNA sequences from the 5'-flanking region of the rat insulin gene. Initial experiments localized isl-1 to the islet cells of the pancreas, suggesting a possible role for this protein in the regulation of insulin gene expression and/or islet cell development. More recent studies have determined that isl-1 is also expressed in the central and peripheral nervous system. We now report that isl-1 gene expression is not restricted to cells of neuroendocrine lineage. Northern blot analysis of rat and hamster fibroblasts, rat keratinocytes, and human epidermoid carcinoma cells detected messenger RNA transcripts that hybridized, at high stringency, to different isl-1 complementary DNA probes. DNA sequence analysis of human and hamster isl-1 complementary DNAs generated by reverse transcription-polymerase chain reaction from nonneuroendocrine cell lines demonstrated 100% conservation of isl-1 amino acid sequences from rat hamster and human species. Western blot analysis with isl-1 antiserum demonstrated that immunoreactive isl-1 was detectable in nuclear extracts from islet cell lines. In contrast, immunoreactive isl-1 was only detected in cytoplasmic extracts from nonneuroendocrine cell lines. The results of these studies demonstrate striking conservation of mammalian isl-1 coding sequences and widespread expression of the isl-1 gene in heterologous cells. These experiments suggest that the biological importance of isl-1 may not be restricted to neuroendocrine cell lineages and raise the possibility that isl-1 function may be regulated by sequestration in distinct cellular compartments.
M Wang; D J Drucker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  134     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-04-07     Completed Date:  1994-04-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1416-22     Citation Subset:  AIM; IM    
Department of Clinical Biochemistry, Toronto General Hospital, University of Toronto, Ontario, Canada.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/S69329;  S70720;  S70721
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cells, Cultured
Conserved Sequence
DNA, Complementary / chemistry*
DNA-Binding Proteins / analysis,  genetics*,  physiology
Gene Expression
Genes, Homeobox*
Homeodomain Proteins*
Molecular Sequence Data
Nerve Tissue Proteins*
RNA, Messenger / analysis
Tumor Cells, Cultured
Reg. No./Substance:
0/DNA, Complementary; 0/DNA-Binding Proteins; 0/Homeodomain Proteins; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/insulin gene enhancer binding protein Isl-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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