| LH responses to LHRH in perifused pituitary cell culture: sex differences in the rat. | |
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MedLine Citation:
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PMID: 6364840 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present experiments were designed to study male and female luteinizing hormone (LH) secretory patterns following pulsatile or continuous LH-releasing hormone (LHRH) administration using a perifused dispersed rat anterior pituitary cell culture system. In male cells, consistent LH responses were elicited by hourly LHRH pulses (30 pmol), whereas in the female cells (proestrus or diestrus I), a statistically significant (P less than or equal to 0.01) decrease in the LH secretion occurred with successive LHRH pulses. Proestrous cells secreted significantly (P less than or equal to 0.01) more LH than diestrous cells during the first 24 h but not after 48 h in culture. When exposed to a 6-h continuous infusion of LHRH (10 nM), male cells released LH in a single phase and female cells secreted LH in a biphasic pattern. These data suggest that significant sex differences exist in the rat LH secretory pattern elicited by LHRH in vitro. |
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Authors:
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J S Loughlin; P G Naddaff; T M Badger |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 246 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1984 Feb |
Date Detail:
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Created Date: 1984-03-16 Completed Date: 1984-03-16 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E145-52 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Estrus Female Gonadotropin-Releasing Hormone / pharmacology* Luteinizing Hormone / secretion* Male Pituitary Gland, Anterior / cytology*, secretion Pregnancy Radioimmunoassay Rats Rats, Inbred Strains Sex Factors Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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HD-06270/HD/NICHD NIH HHS; HD-15788/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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33515-09-2/Gonadotropin-Releasing Hormone; 9002-67-9/Luteinizing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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