Document Detail

The LFA-1 antigen in human B lymphocyte activation.
MedLine Citation:
PMID:  2659238     Owner:  NLM     Status:  MEDLINE    
Human tonsil B cells include a subpopulation (30 per cent) of cells which lack LFA-1 antigen. Activation of tonsil B cells by culture with anti-IgM and interleukin-4 led to an increase in staining intensities and in the proportion of cells staining, until by 48 h the majority of B cells were positive. Culture of activated cells with low-molecular weight B cell growth factor, which induces a proportion of cells to proliferate, led to a minor further increase in expression of the LFA-1 antigen. Inclusion of a monoclonal antibody against the LFA-1 beta chain in culture did not affect either proliferation or immunoglobulin secretion. The expression of LFA-1 by B cells thus changes as B cells are activated, perhaps reflecting the changing requirements of B cells for interaction with other cells and tissue components. On the other hand, our results did not provide any support for the idea that the LFA-1 antigen is directly involved in the interaction of B cells with lymphokines which control proliferation and differentiation.
H Zola; V Furness; A Nikoloutsopoulos; S H Neoh; S Barclay; R Starr; A Day; G R Russ
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Disease markers     Volume:  7     ISSN:  0278-0240     ISO Abbreviation:  Dis. Markers     Publication Date:    1989 Apr-Jun
Date Detail:
Created Date:  1989-07-27     Completed Date:  1989-07-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8604127     Medline TA:  Dis Markers     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  95-104     Citation Subset:  IM    
Department of Clinical Immunology, Flinders Medical Centre, Flinders University of South Australia, Bedford Park.
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MeSH Terms
Antibodies, Monoclonal / diagnostic use
Antibody Formation
Antigens, Differentiation / analysis*
B-Lymphocytes / drug effects,  immunology*
Cell Survival / drug effects
Cells, Cultured
Interleukins / pharmacology
Lymphocyte Activation*
Lymphocyte Function-Associated Antigen-1
Membrane Glycoproteins / analysis*
Palatine Tonsil / immunology
Spectrometry, Fluorescence
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Differentiation; 0/Interleukin-6; 0/Interleukins; 0/Lymphocyte Function-Associated Antigen-1; 0/Membrane Glycoproteins

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