| LET dependency of heavy-ion induced apoptosis in V79 cells. | |
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MedLine Citation:
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PMID: 11037583 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated the relationship between the LET values and cell death, defined as either apoptosis or loss of reproductive integrity (reproductive death), using Chinese hamster V79 cells. The cells were irradiated with X-rays or carbon-ion beams from the Heavy Ion Medical Accelerator in Chiba (HIMAC) at the National Institute of Radiological Sciences (NIRS). Apoptosis was defined based on the morphological change upon treating of cells with caffeine. The apoptotic index, the ratio of apoptotic cells to the total, after exposure to 2 Gy of X-rays was 0.043. Upon irradiation with carbon-ion beams, the index was gradually increased with increasing LET values, reaching a maximum of 0.076 at 110 keV/micron, and then decreased to 0.054 at 237 keV/micron. An analogous pattern of the LET dependence was observed between reproductive death and apoptotic death. The cell-survival values obtained after 2 Gy exposure (SF2) were 0.64, 0.13, and 0.24, respectively. A similar trend was found for the RBE values calculated from the initial slope for both apoptosis and reproductive death. These results strongly suggest that the target for both types of cell death is the same. |
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Authors:
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M Aoki; Y Furusawa; T Yamada |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of radiation research Volume: 41 ISSN: 0449-3060 ISO Abbreviation: J. Radiat. Res. Publication Date: 2000 Jun |
Date Detail:
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Created Date: 2000-11-20 Completed Date: 2000-11-20 Revised Date: 2012-03-28 |
Medline Journal Info:
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Nlm Unique ID: 0376611 Medline TA: J Radiat Res Country: JAPAN |
Other Details:
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Languages: eng Pagination: 163-75 Citation Subset: IM |
Affiliation:
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Space and Particle Radiation Science Research Group, National Institute of Radiological Sciences, Chiba, Japan. mizuho@nirs.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / radiation effects* Caffeine / pharmacology Carbon / adverse effects* Cell Cycle / drug effects, radiation effects Cell Division / radiation effects Cell Line / radiation effects Chromatin / drug effects, radiation effects, ultrastructure Colony-Forming Units Assay Cricetinae Cricetulus DNA / radiation effects DNA Damage DNA Fragmentation Dose-Response Relationship, Radiation Fibroblasts / drug effects, radiation effects*, ultrastructure Heavy Ions / adverse effects* Linear Energy Transfer* Radiation-Sensitizing Agents / pharmacology Relative Biological Effectiveness X-Rays |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Radiation-Sensitizing Agents; 58-08-2/Caffeine; 7440-44-0/Carbon; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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