Document Detail

LET-99 determines spindle position and is asymmetrically enriched in response to PAR polarity cues in C. elegans embryos.
MedLine Citation:
PMID:  12223405     Owner:  NLM     Status:  MEDLINE    
Asymmetric cell division depends on coordinating the position of the mitotic spindle with the axis of cellular polarity. We provide evidence that LET-99 is a link between polarity cues and the downstream machinery that determines spindle positioning in C. elegans embryos. In let-99 one-cell embryos, the nuclear-centrosome complex exhibits a hyperactive oscillation that is dynein dependent, instead of the normal anteriorly directed migration and rotation of the nuclear-centrosome complex. Furthermore, at anaphase in let-99 embryos the spindle poles do not show the characteristic asymmetric movements typical of wild type animals. LET-99 is a DEP domain protein that is asymmetrically enriched in a band that encircles P lineage cells. The LET-99 localization pattern is dependent on PAR polarity cues and correlates with nuclear rotation and anaphase spindle pole movements in wild-type embryos, as well as with changes in these movements in par mutant embryos. In particular, LET-99 is uniformly localized in one-cell par-3 embryos at the time of nuclear rotation. Rotation fails in spherical par-3 embryos in which the eggshell has been removed, but rotation occurs normally in spherical wild-type embryos. The latter results indicate that nuclear rotation in intact par-3 embryos is dictated by the geometry of the oblong egg and are consistent with the model that the LET-99 band is important for rotation in wild-type embryos. Together, the data indicate that LET-99 acts downstream of PAR-3 and PAR-2 to determine spindle positioning, potentially through the asymmetric regulation of forces on the spindle.
Meng-Fu Bryan Tsou; Adam Hayashi; Leah R DeBella; Garth McGrath; Lesilee S Rose
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  129     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-09-11     Completed Date:  2002-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  4469-81     Citation Subset:  IM    
Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.
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MeSH Terms
Caenorhabditis elegans / embryology,  genetics
Caenorhabditis elegans Proteins / genetics,  metabolism*
Cell Lineage
Cell Nucleus / metabolism
Mitotic Spindle Apparatus / physiology*
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/LET-99 protein, C elegans; 0/PAR-3 protein, C elegans; 0/par-2 protein, C elegans

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