Document Detail


LDL-induced cytotoxicity and its inhibition by HDL in human vascular smooth muscle and endothelial cells in culture.
MedLine Citation:
PMID:  223585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human aortic medial smooth muscle cells (SMC) and umbilical vein endothelial cells (EC) in culture were exposed to various concentrations of plasma low density (LDL) and high density (HDL) lipoproteins prepared from normolipemic donors in order to assess their effects on cell growth. So that the effects of each lipoprotein could be evaluated separately and in combination, lipoproteins were added to culture medium containing lipoprotein deficient serum (LPDS, d greater than 1.25 g/ml at a protein concentration of 4.5 mg/ml of medium). The addition of LDL at cholesterol concentrations of 160 microgram/ml of culture medium, resulted in significant reductions in both the number of SMC and EC cells per dish within 3 days of exposure (P less than 0.001, SMC; P less than 0.01, EC), when compared with LPDS controls and the starting cell numbers. This cytotoxic phenomenon was dose-related, and only at LDL cholesterol concentrations equal to or below 50 microgram/ml were no marked changes observed. In contrast, HDL at all concentrations tested produced no such deleterious effects. Autoradiographic assessment of DNA synthesis confirmed these findings. After 48 h of continuous exposure to tritiated thymidine, labeling indexes reached much lower plateaus in the LDL-treated groups.
Authors:
J R Hessler; A L Robertson; G M Chisolm
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Atherosclerosis     Volume:  32     ISSN:  0021-9150     ISO Abbreviation:  Atherosclerosis     Publication Date:  1979 Mar 
Date Detail:
Created Date:  1979-09-17     Completed Date:  1979-09-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  213-29     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aorta, Thoracic / cytology,  drug effects
Autoradiography
Cell Count / drug effects
Cell Survival / drug effects
Cholesterol / pharmacology
Culture Techniques
Endothelium / cytology,  drug effects*
Humans
Infant
Infant, Newborn
Lipoproteins, HDL / blood,  pharmacology*
Lipoproteins, LDL / blood,  pharmacology*
Muscle, Smooth / cytology,  drug effects*
Umbilical Veins / cytology,  drug effects
Chemical
Reg. No./Substance:
0/Lipoproteins, HDL; 0/Lipoproteins, LDL; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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