| LDL-apheresis depletes apoE-HDL and pre{beta}-1 HDL in Familial Hypercholesterolemia : Relevance to atheroprotection. | |
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MedLine Citation:
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PMID: 21957200 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Subnormal HDL-cholesterol and apolipoprotein AI (apoAI) levels are characteristic of Familial Hypercholesterolemia (FH), reflecting perturbed intravascular metabolism with compositional anomalies in HDL particles including apolipoprotein E (apoE) enrichment. Does LDL-apheresis, which reduces HDL-cholesterol, apoAI and apoE by adsorption, induce selective changes in HDL subpopulations, with relevance to atheroprotection?Five HDL subpopulations were fractionated from pre and post LDL apheresis plasmas of normotriglyceridemic FH subjects (n=11) on regular LDL apheresis (>2 years). Apheresis lowered both plasma apoE (-62%) and apoAI (-16%) levels, with preferential, genotype-independent, reduction in apoE. The mass ratio of HDL2:HDL3 was lowered from ≈1:1 to 0.72:1 by apheresis, reflecting selective removal of HDL2 mass (80% of total HDL adsorbed). Pre-LDL apheresis, HDL2 subpopulations were markedly enriched in apoE, consistent with ≈1 copy of apoE per 4 HDL particles. Large amounts (50-66%) of apoE-HDL were removed by apheresis, preferentially in the HDL2b subfraction (-50%); minor absolute amounts of apoE-HDL were removed from HDL3 subfractions. Furthermore, pre-β1-HDL particle levels were subnormal following removal (-53%) upon apheresis, suggesting that cellular cholesterol efflux may be defective in the immediate post-apheresis period. In LDL receptor deficiency, LDL-apheresis may enhance flux through the reverse cholesterol transport pathway, and equally attenuate potential biglycan-mediated deposition of apoE-HDL-cholesterol in the arterial matrix. |
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Authors:
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Alexina Orsoni; Samir Saheb; Johannes H M Levels; Geesje Dallinga-Thie; Marielle Atassi; Randa Bittar; Eric Bruckert; Anatol Kontush; Alain Carrie; M John Chapman |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-26 |
Journal Detail:
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Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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INSERM U939 / UPMC, France; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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