Document Detail


LD typing in man using cells frozen and stored in microtiter plates.
MedLine Citation:
PMID:  50649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method of freezing LD typing cells in microtiter plates was developed. Two times 10(5) lymphocytes in a small volume (15 mul) with a low DMSO concentration (5%) were directly frozen in a --70 degrees C freezer. These cells were used in the mixed lymphocyte culture after thawing by diluting step-by-step with serum and culture medium without washing. The kinetics obtained with cells frozen in microtiter plates were comparable to those with fresh cells. Typing for LD determinants was performed in a group of unrelated persons in a family study using the LD homozygous reference cell Pi frozen in microtiter plates.
Authors:
B Netzel; H Grosse-Wilde; P Baumann; W Mempel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tissue antigens     Volume:  6     ISSN:  0001-2815     ISO Abbreviation:  Tissue Antigens     Publication Date:  1975 Jul 
Date Detail:
Created Date:  1975-11-07     Completed Date:  1975-11-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0331072     Medline TA:  Tissue Antigens     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  8-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Epitopes*
Freezing
Histocompatibility Testing / methods*
Homozygote
Humans
Lymphocyte Culture Test, Mixed
Lymphocytes / immunology*
Preservation, Biological
Chemical
Reg. No./Substance:
0/Epitopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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